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Title:	Accumulation of potato virus y- in nicotiana tabacum callus culture to obtain a virus preparation
Authors:	Khassanov V. T Beisembina B Fida M. A
Keywords:	Potato virus Y- (PVY) Callus tissue ELISA Antigen Polyclonal antibodies
Issue Date:	Feb-2016
Description:	The accumulation of potato virus Y- (PVY) in tissue culture of Nicotiana tabacum (N. tabacum) was studied. Plants of N. tabacum variety, Samsun, inoculated with PVY-infected sap of potato variety, Cherie, were used for the virus accumulation. According to enzyme-linked immunosorbent assay (ELISA) results, virus showed the highest optical density (OD) on the 25th day of inoculation. Murashige and Skoog medium containing kinetin 2mg/l, 2.4-D 0.5 mg/l, indole-acetic acid 1 mg/l sucrose 2% agar 0.7%, in addition to the standard components, was used to induce callus culture from N. tabacum leave explants. ELISA results showed that the callus culture was able to maintain viral infection during four transplantations. Slightly and highly purified (Y-Cherie) virus preparations were obtained from the PVY-infected tissue culture. The slightly-purified antigens showed an OD approximately equal to the positive control in sandwich ELISA. The Y-Cherie antigen was detected as PVY necrotic strain. Specific to the virus polyclonal antibodies that reacted with a maximum 1/3200 titer of antigen in indirect ELISA were obtained in the result of the laboratory mouse immunisation.
News Source:	Pertanika Journals
ISSN:	1511-3701
Volume:	39
Pages:	145-153
Publisher:	Universiti Putra Malaysia
URI:	https://ptsldigital.ukm.my/jspui/handle/123456789/576857
Appears in Collections:	Journal Content Pages/ Kandungan Halaman Jurnal

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