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https://ptsldigital.ukm.my/jspui/handle/123456789/519869| Title: | Metagenomics approach in understanding plant microbial diversity and its indication in bioprospecting of antibiotic producing bacteria |
| Authors: | Haruna Emmanuel (P73270) |
| Supervisor: | Noraziah M. Zin, Dr. |
| Keywords: | Metagenomics Microbial Dissertations, Academic -- Malaysia |
| Issue Date: | 26-Nov-2017 |
| Description: | The advent of Metagenomics which is a culture-independent method has provided opportunities to study microbial community in situ. The method provides a true insight of most habitats bacterial community compositions and phylogenetic patterns (randomized or niche-specific). The biological implications of these patterns on how it could affect efficient sampling of diverse bacterial species producing antibiotics from different plant species and parts have never been studied. In this study, metagenomics method was employed to understand the bacterial community pattern of three ethnomedicinal plant species (Santiria apiculata, Microdesmis caseariifolia, and Rothmannia macrophylla) parts (leaves, stems, roots and rhizospheric soil). The phylogenetic pattern revealed by this approach was validated using the culturedependent method to ascertain how diverse would be the anti-ESKAPE bacterial isolated from the three-plant species parts and ten replicates of Santiria stem using dilute tryptic soy agar and water agar media. The metagenomic analysis revealed a randomized bacterial phylogenetic pattern, implying no niche-specificity. Further analysis showed that this pattern is governed by a stochastic process. The implication of this pattern in bioprospecting revealed that more diverse bacterial species would be obtained in culture if more replicates of same plant species and same part are sampled than when different plant species and parts are considered from all samples collected. This prediction was in congruence with the culture-dependent method with a high and diverse yield of anti-ESKAPE bacteria (n = 53) from the ten replicates of Santiria stem compared to the three-plant species parts (n = 30), which yielded more similar anti- ESKAPE bacterial species. Among the many anti-ESKAPE isolates obtained, SUK123 (Paenibacillus tyrfis) crude extract was active against ATCC MRSA and its clinical strains. Its metabolomics profile revealed the presence of some antibacterials such as Cefditoren pivoxil, Celastramycin A, and Meclocycline. Its draft genome size was 8.04 Mbs with hypothetical and non-hypothetical coding proteins and secondary metabolites biosynthetic genes clusters. Aside the novel bacteria isolated, this study discovered that UKM rainforest plants are associated with a non niche-specific bacteria. This bacterial pattern should be considered subsequently for efficient sampling of diverse bacterial species that are antibiotics producers.,Doktor Falsafah |
| Pages: | 204 |
| Call Number: | QW52.E54m 2017 9 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Health Sciences / Fakulti Sains Kesihatan |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_99843+Source01+Source010.PDF Restricted Access | 3.89 MB | Adobe PDF |  View/Open |
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