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https://ptsldigital.ukm.my/jspui/handle/123456789/519658| Title: | Self assembled amino acid surfactant-based vesicle and micromulsion gel as promising carriers for drug delivery |
| Authors: | Nasima Akter (P48279) |
| Supervisor: | Shahidan Radiman, Prof. Dr. |
| Keywords: | Sodium N-lauroylsarcosinate hydrate (SNLS) Amino acid based biosurfactants Universiti Kebangsaan Malaysia -- Dissertations |
| Issue Date: | 13-Dec-2012 |
| Description: | Sodium N-lauroylsarcosinate hydrate (SNLS) is an amino acid based biosurfactants. They are biodegradable, nontoxic, comparatively cheaper and achievable from renewable natural sources. Moreover, the amino acid moiety in the polar head group area gives it a surprising potential to interact with both cationic surfactant and anionic polyelectrolyte. Such fascinating properties were exploited in preparing vesicle, microemulsion and kappa carrageenan based microemulsion gel. The ternary phase behaviour of sodium N-lauroylsarcosinate hydrate/1-decanol/water was examined in the water rich corner (90-99.96% water). Varieties of aggregate structures were achieved by changing the mixing ratio of both amphiphiles for the same concentration of water. In both amphiphile-water boundary two single microemulsion phases were found and in intermediate region a single vesicle phase was found (between the molar ratios of 5:6.5 and 1:4 in 95-90 wt% water). For molar ratios of 8:5-5:6.5 and 1:4- 1:8.5 of SNLS/1-decanol, the solution remained two phases. The macroscopic appearance of the lower phase changed from clear to milky white with increasing concentrations of 1-decanol. Both microemulsion phases were revealed non viscous and isotropic. From one morphological study, it was observed that vesicle formation depended on the formation path. Two different mixing procedures: surfactant mixed with water followed by 1-decanol gives lamellar structure; another surfactant mixed with 1-decanol followed by water gives vesicle structure. Another microemulsion phase was investigated using the same surfactant with oleic acid as an oil phase. This microemulsion phase was immobilized into kappa carrageenan gel network to facilitate oral or transdermal drug delivery. All of the samples were studied by visual observation and using, cross polarizer, light microscopy, transmission electron microcopy (TEM), dynamic light scattering (DLS), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR). A model drug "Ibuprofen" was used for drug delivery study. Ibuprofen is an orally administered, non steroidal anti-inflammatory agent used extensively in the treatment of arthritis and also shows significant antiproliferative activity against mouse colorectal cancer. Ibuprofenmicroemulsion gel complex has shown an initial burst release followed by a controlled release. The burst release is the most favorable mechanism of delivery in some instances. At the beginning of wound treatment, an initial burst provide an immediate relief followed by prolonged release to promote gradual healing. On the other hand, the drug-vesicle showed a sustained release of ibuprofen for a period of 28 hour without any burst. This pattern of release profile is one of the important features in drug delivery because it is sustained for an extended period of time in biological environment. The probable cytotoxicity effect of vesicles and microemulsion gel were evaluated on L929 fibroblast cells, treated for 24 hour at concentrations ranging 0- 5000 μg/ml. Both of the systems were found nontoxic even at higher concentration. An antiproliferative activity of ibuprofen-vesicle and ibuprofen-microemulsion gel was verified against jargot cancer cell lines in vitro study. The inhibition rate is significantly higher of both of the systems in comparison to the free drug,Certification of Master's/Doctoral Thesis" is not available |
| Pages: | 207 |
| Call Number: | QD506.A394 2012 tesis |
| Publisher: | UKM, Bangi |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi |
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