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Title:	In vitro induction of local rice varieties, MRQ74 & MR269 plant salt tolerance and assessment of variability through biochemical & molecular marker
Authors:	Arshad Naji Husin Alhasnawi (P75248)
Supervisor:	Che Radziah Che Mohd Zain, Prof. Dr.
Keywords:	In vitro technique Local rice Biochemistry
Issue Date:	22-Jun-2017
Description:	Salinity is one of the main issues encountered in interrupting the metabolic processes of plants, thus limiting crop productions. The primary objective of this study was to establish and develop regeneration protocols (direct and indirect plant regeneration) to elicit salt tolerance in local rice varieties MRQ74 and MR269, using an in vitro selection technique followed by the assessment of genetic variability using an inter-simple sequence repeat (ISSR) marker. In the present investigation, 200 mM NaCl was applied to produce salinity stress conditions within the growth medium. An in vitro experiment was conducted to evaluate the potential of exogenous application at different concentrations of ascorbic acid (AsA) and polysaccharide from mushroom (beta glucan, BG) to alleviate the effect of stress conditions on seedlings and callus of both rice varieties. There was selection for the higher levels of antioxidant enzyme and proline activities in seedling and callus for plant regeneration (direct and indirect) and then transfer to a glasshouse for the hardening and acclimatization process to produce generation (F1) plants. To verify the stability of the acquired salinity tolerant plants, selected F1 seeds from both sources (direct and indirect regeneration) then were germinated under irrigation salinity stress in glasshouse for F2 generation plants. The same process was carried out for these F2 generation selected plants to produce F3 generation tolerant plants. Seedlings from F3 generation then were selected together with seedlings from its mother plant. Molecular evaluations were done to determine DNA polymorphism of the selected variance derived from MRQ74 and MR269 variety for salt tolerance via ISSR markers. Results showed that seedling and callus of both varieties exposed to salt stress exhibited was significant reductions in growth characteristics. The findings from the treatment using AsA and BG revealed enhancement of growth characteristics, antioxidant enzymes, and proline activities. These, all leading to improve process plant regeneration to yield F1 generation plants from direct and indirect sources. The F2 generation plants from both sources (direct and indirect) acquired under irrigation salinity stress and the results showed that F2 generation plants derived from indirect plant regeneration (callus sources) had a superior phenotype as well as physiological and chemical characteristics. Germination of generation F2 seeds resulted in the production of generation F3 seedlings derived from indirect (callus sources) that were then were chosen for genetic variation assessment based on polymorphisms among the selected samples for both MRQ74 and MR269. Assessments of genetic variability through ISSR marker revealed significant differences between controls and those treated with AsA and BG. These findings are expected to contribute in understanding of the relationship between the physiological and anatomical structure as well as molecular data for epigenetic regulation of salinity tolerance in both rice varieties for developing new traits to enhance tolerance in Malaysian rice varieties.,Certification of Master's/Doctoral Thesis" is not available
Pages:	293
Call Number:	QD415.A349 2017 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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