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Title:	Effects of temperature and diet on growth and physiology of hybrid grouper Epinephelus fuscoguttatus ♀ × E. Lanceolatus ♂
Authors:	Moumita De (P64829)
Supervisor:	Mazlan Abd. Ghaffar, Prof. Dr.
Keywords:	Temperature Diet Grouper species Groupers
Issue Date:	30-Jun-2016
Description:	The commonly cultured grouper species, the giant grouper (Epinephelus lanceolatus) and tiger grouper (Epinephelus fuscoguttatus) are enlisted as vulnerable species in IUCN catalogue. These native species are getting less popular in mariculture because of their slow growth rate. Through the hybridization of this local species (E. lanceolatus ♂ × E. fuscoguttatus ♀) a new strain of grouper (Tiger grouper giant grouper hybrid: TGGGH) is introduced. Elevated seawater temperature, and diets can affects growth, physiological and biochemical indices of ectotherm animals like fish. The objectives of this study were to determine the length weight relationship (LWR), condition, food consumption (FC), food conversion (FCR), specific growth rate (SGR), gastric emptying time and rate (GET and GER), pepsin enzyme activity, haematology and biochemical indices and oxygen consumption rate (OCR) and gill fine structure of TGGGH at different temperatures (22, 26, 30 and 34°C) and diets (commercial diet: pellet, natural diet: shrimp). The fish were collected from local hatchery of Banting, Selangor, Malaysia. The experiment was conducted in the laboratory using flow through seawater aquaria, and similar size independent experimental tanks. After the one month experiment, TGGGH exhibited negative allometric growth (b < 3) at 22°C and 34°C, isometric growth (b = 3) at 26°C fed on both diet, while positive allometric growth (b > 3) at 30°C fed on shrimp diet. The better condition was observed at 26 and 30oC (K, Kn = 1, > 1) while poor condition (K, Kn = < 1) at 22 and 34oC fed on both diet. FC, FCR, and SGR were significantly influenced by temperature and diet types. ignificantly highest (p < 0.05) mean SGR (1.00% BM day-1) was seen in the 30°C fed on shrimp diet while lowest at 22°C fed pellet diet (0.59% BM day-1) but no significant difference (p > 0.05) in growth was seen between 22 and 34oC fed on both diet. Significantly (p <0.05), lowest FC was observed at 22oC while significantly (p < 0.05) highest FCR (1.208) was seen at 22oC fed on both diet. The fastest gastric emptying was seen at 30°C fed on shrimp (12 h) diet, while a significant delay in gastric empting was observed at 22°C fed on pellet diet (16 h). The digestion rate of TGGGH also incorporated using modified square root model [St = S0 (1-S0 (α-1) ρ (1-α) t) 1/ (1- α) + ζ] for all temperatures and diets. The highest pepsin enzyme activity was observed at 30oC fed on shrimp diet (6.28 SU mg g-1) while lowest at 22oC (1.68 SU mg g-1) fed on pellet diet. Likewise at 30oC TGGGH exhibited better haematological indices. However, changes in diet only affect haematology indices, failed to elicit changes in biochemical indices. OCR increased significantly (p < 0.05) from 0.06 to 0.11 ml O2 g-1h-1 with increasing temperature. The final preferred temperature estimated from the temperature quotients (Q10 = 1.16) was between 26 and 30oC. However no clear differences of gill fine structure were observed for TGGGH at all temperature ranges by scanning electron microscope. The result indicate that 22 and 34oC present a particular challenge to TGGGH aquaculture and 30oC exhibits the best growth performance and fastest digestion and conditions followed by 26oC which suggests that 26 and 30oC can be measured as optimum temperature for the aquaculture of this newly developed fish species feeding either shrimp or pellet diet. The data obtained from this study would be useful for fish biologist and aquaculture managers for optimization of massive production of this newly developed fish species.,Certification of Master's/Doctoral Thesis" is not available
Pages:	186
Call Number:	QL638.S48M638 2016 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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