1. UKM Learning and Research Repository
2. PHD Thesis / Tesis PHD
3. Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

Title:	Development and surface funtionalisation of palm oil-based cross-linked block copolyesters nanoparticles for usage in a targeted drug delivery system
Authors:	Rida Tajau (P80625)
Supervisor:	Rosiah Rohani, Assoc. Prof. Ts. Dr.
Keywords:	Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia Palm oil Nanoparticles
Issue Date:	11-Dec-2020
Description:	Synthetic and high molecular weight (MW) polymer-based nanoparticles (NPs) for drug delivery have a high tendency to cause immunologic inflammatory responses due to their lower biodegradability and higher cytotoxicity in comparison to natural-based low MW polymers. Natural-based low MW polymers have the potential to overcome these problems and their development from palm oil sources have been revolutionised, due to the abundance of palm oil resources. Therefore, this study was conducted to develop and surface functionalise low MW palm oil-based cross-linked block copolyester NPs for targeted drug delivery systems. These NPs were derived from the copolyester of acrylated palm olein and polyol ester. The copolyester NPs were initially generated via gamma radiation-induced reversible addition-fragmentation chain transfer polymerisation and cross-linking techniques. The obtained NPs were of positive charged and spherical shape with particle diameter of less than 200 nm as measured by the zeta potential analyser, dynamic light scattering, Transmission Emission Microscopy and Field Emission Scanning Electron Microscopy. Result from Gel Permeation Chromatography (GPC) analysis confirmed that the obtained NPs had a low average MW (24 kDa) and a controlled MW distribution with a narrow polydispersity index of 1.01. The hydrolysed ester bond of these NPs possessed excellent biodegradable properties as verified by Fourier Transform Infrared (FTIR) and Neutron Magnetic Resonance (NMR). Successful surface functionalisation of the NPs via succinylation and N-hydroxysuccinimide activated technique was obtained with nearly 90% peptide functionalisation and 100% paclitaxel entrapment. Analysis with FTIR, NMR, GPC, X-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry further affirmed that the NPs possessed biodegradable hydrolysed ester chemical structures, low MW (<100 kDa), amorphous characteristic, and good thermal properties. Drug release kinetic study conducted found that the NPs followed matrix-controlled diffusion release system, which obeyed the first-order drug release kinetic. The high-content screening image study on the other hand confirmed that the Michigan Cancer Foundation-7 (MCF-7) cells were successfully localised into the fluorescent-labelled and paclitaxel-loaded NPs. In conclusion, the new palm oil-based peptide-targeted NPs were successfully developed with full potential for targeted drug delivery applications. This is due to their high drug loading and encapsulation efficiency, high drug controlled release efficiency profiles, and ability to target cancer cells specifically. From this study, the formation of spherical dual-functionality (peptide-targeted and paclitaxel-therapeutic) NPs showed a remarkable cell viability (between 5 and 20%) for the inhibition of MCF-7 cells using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.,Ph.D.
Pages:	296
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.