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Title:	Microalgae isolation and cultivation for CO2 fixation using palm oil mill effluent
Authors:	Ding Gong Tao (P68071)
Supervisor:	Zahira Yaakob, Prof. Ir. Dr.
Keywords:	Microalgae Palm oil Algal biofuels
Issue Date:	1-Apr-2017
Description:	Palm oil industry is one of the most significant agro-industry in Malaysia, and it contributes a large portion of the country's gross domestic product (GDP). Approximately 503-755 million m3 of CO2 is produced due to offgas emission from the treatment of anaerobic digestion of palm oil mill effluent (POME) in Malaysia in 2015, which could lead to global warming. Additionally, in 2015 Malaysian palm oil mills generate approximately 49.9-74.9 million tons of POME which contains high concentrations of nutrients. This thesis deals with the isolation, characterization and identification of the native microalgal species from POME in Malaysia, the determination of the concentration of assimilable organic carbon in POME from different ponds, the potential of POME as cultivation medium for autotrophic metabolic growth of native microalgae, the determination of the dominant microalgal species for CO2 fixation using POME, the determination of the effect of dissolved CO2 concentration (% v/v) on microalgal biomass growth, the evaluation of the CO2 fixation rate and light intensity in the batch and semi-continuous cultivation of microalgae in the pilot-scale photobioreactor. Spread plate technique was applied to isolate and purify the microalgal strains in single colonies. Furthermore, morphological characteristics and genomics analysis were used to identify the microalgal species. High performance liquid chromatography (HPLC) was used to screen sugars and volatile fatty acids in POME which were considered as potential microalgal-assimilable organic carbons, to select the optimum POME source for CO2 fixation. Acetic acid was added as microalgal assimilable carbon to the POME which does not contain assimilable carbon and used to cultivate the microalgae to study the effect on their growth. Microalgae biomass concentration was determined in the cultivation in POME as medium at different CO2 concentrations, and the maximum specific growth rate (μmax) of microalgae and maximum CO2 fixation rate (Rco2) were calculated. Chlorella sp. UKM2 and Chlamydomonas sp. UKM6 were isolated from POME of Sime Darby East Palm Oil Mill. Coelastrella sp. UKM4 was isolated from POME of the mill of Dominion Square Sdn Bhd. Chlorella sp. UKM7 was isolated from POME of Sime Darby Sua Betong Mill. The concentration of acetic acid and propionic acid in anaerobic POME from Sime Darby Sua Betong Mill was found to be 185 mg L-1 and 230 mg L-1, respectively. Chlorella sp. UKM2, Chlamydomonas sp. UKM6 and Chlorella sp. UKM7 are mixotrophic microalgal species. The μmax of these three species increased significantly when acetic acid was added into the POME. Chlorella sp. UKM2 showed the highest dominance in cell count in the mixed culture and has the highest Rco2 at both 0.04% and 1% of the CO2 concentrations, and was chosen. In the study of effect of CO2 concentration (v/v%) on the microalgae growth and CO2 fixation in 2 L conical flask, 0.5% of CO2 concentration in the sparging gas was required to achieve the optimal μmax and Rco2 of 1.032 day-1 and 373.1 mg L-1 day-1. In the batch growth in PBR, Chlorella sp. UKM2 exhibited the μmax and Rco2 of 1.183 day-1 and 455.3 mg L-1 day-1, respectively. In the semi-continuous growth of two phases, the average CO2 fixation rate presented 471.0 mg L-1 day-1 and 600.5 mg L-1 day-1, respectively.,Certification of Master's/Doctoral Thesis" is not available
Pages:	145
Call Number:	TP339.D537 2017 3 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

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