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https://ptsldigital.ukm.my/jspui/handle/123456789/485636| Title: | Simvastatin-loaded alginate hydrogel films in diabetic wound healing |
| Authors: | Masoud Rezvanian (P73187) |
| Supervisor: | Shiow-Fern Ng, Assoc. Prof. Dr. |
| Keywords: | Angiogenesis Lymphangiogenesis Diabetes Dissertations, Academic -- Malaysia |
| Issue Date: | 20-Mar-2018 |
| Description: | Inadequate blood supply is one of the common clinical signs in chronic wounds and this has become the major hindrance for a chronic wound to heal. Recently simvastatin has shown to promote angiogenesis and lymphangiogenesis in diabetic mice when applied topically, suggesting an important implication for patients with diabetic wound. However, topical application of simvastatin in the conventional topical formulation such as ointment is not suitable due to short residence time. Hence, the aim of this study was to develop a modern wound dressing using natural biopolymers to deliver simvastatin in a sustained release manner for diabetic chronic wounds. The hydrocolloid films were developed using the blend of sodium alginate (SA), gelatine (GL) and pectin (PC) to obtain SA, SA-GL and SA-PC and then characterized by various means. In the optimisation study, SA-PC hydrocolloid films were chemically crosslinked with CaCl2 solution (0.5–3% w/v) and further tested. The results revealed no physicochemical interactions and SA-PC gave superior physical profile with a slower release of drug. Hence SA-PC film loaded with simvastatin was selected for further optimization. From the optimisation studies, crosslinking had enhanced wound fluid uptake ability of film with the best result for 0.5% CaCl2 concentration. Thermal analysis revealed that the crosslinking process increased the thermal stability and delivered simvastatin in a slow sustained manner. In vitro cytotoxicity assay demonstrated the developed formulation was biocompatible to the skin fibroblast cells. Finally, in vivo studies were performed on diabetic Wistar rats for the period of 21-days. Wound healing rate, haematology, histology, collagen and VEGFA measurement were obtained. The animal studies revealed that the wound dressing healed the wounded area significantly (p < 0.05) higher than the controls after 21-day treatment and wound closure was ~99% without any adverse systemic reactions to the animals. It was observed that the rate of collagen synthesis and angiogenesis improved in the group treated with the crosslinked hydrogel film loaded with simvastatin. All in all, the findings suggested that SA-PC composite hydrogel film loaded with simvastatin hold a great potential for the acceleration of diabetic wounds and this could further be studied for human use.,Doktor Falsafah |
| Pages: | 202 |
| Call Number: | QV20.5.R467s 2018 9 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Pharmacy / Fakulti Farmasi |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_99860+Source01+Source010.PDF Restricted Access | 5.57 MB | Adobe PDF |  View/Open |
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