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Title:	Mating compatibility and fitness of male aedes aepypti ridl ox513A-Mx01 strain aginst wild type Mexico and Malaysian strain under contained semi-field conditions
Authors:	Norhaida Hanum Ahmad Tajudin (P45357)
Supervisor:	Hidayatulfathi Othman, Prof. Madya Dr.
Keywords:	Aedes aegypti -- Malaysia Mosquito -- Malaysia Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	15-Apr-2011
Description:	Releasing Insects Carrying Dominant Lethal Gene (RIDL), offers a novel, safe and environmental friendly method to suppress the population of Aedes aegypti mosquitoes below the disease transmission threshold. The objective of this study is to evaluate the mating competitiveness of male Mexican RIDL Ae. aegypti strain against the untransformed wild type and its mating compatibility against a wild type Malaysian female strain under semi-field conditions. The tests were conducted in a field house which was a fully contained trial facility, simulating the living space of two to four people in Malaysia. Ten RIDL Ae. aegypti males and ten wild type Ae. aegypti males were released into the contained trial facility for eight hours in order to compete with each other to mate with ten wild type Ae. aegypti females. All mosquitoes were collected using a mechanical aspirator. Mated females were allowed to lay eggs and the hatched larvae were screened under a fluorescence microscope for the red fluorescent molecular marker to determine if a RIDL male or wild type male had mated them. The mating competitiveness study has been divided into two parts; the mating competitiveness of male Mexican RIDL (MxRIDL-513A) against male Malaysia wild type (MyWT) and the mating competitiveness of male RIDL (Mx RIDL-513A) against male Mexican wild type (MxWT) and replicates were repeated until sufficient data were collected. It was observed that 45% of the mating was with MxRIDL-513A males and 55% with wild type Malaysian males in the first part which did not show any significant difference between these two strains χ2(1, N= 89) p=0.34. In the second part of this study, 54% of females mated with MxRIDL-513A males and the remaining 46% mated with wild type Mexican male. Thus it did not show any significant difference between these two strains χ2(1, N= 58) p=0.59. The results also showed that there was no significant difference (z = -0.586, p=0.56) and (z = -0.966, p=0.33) in the number of eggs produced by female mosquitoes in the first and second part of the study, respectively. No significant difference (z = -0.116, p=0.91) and (z = -606, p=0.54) on the egg hatching rate between the progeny which was fertilized by MxRIDL-513A strain and wild type strain in the first and second part of study, respectively in order to determine the fitness parameters. These result also indicated high mating compatibility of RIDL males with Mexico background and lab-reared Malaysian wild type females. In conclusion, RIDL males are equally competitive and compatible with the wild type males.,“Certification of Master's/Doctoral Thesis” is not available,Master of Health Science
Pages:	97
Call Number:	QX525.N8250m 2011 9 tesis
Publisher:	UKM, Kuala Lumpur
Appears in Collections:	Faculty of Health Sciences / Fakulti Sains Kesihatan

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