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Title:	Human dentine sialophosphoprotein detection using elisa and absorption spectroscopy in root resorption
Authors:	Teh Yongxian (P91615)
Supervisor:	Rohaya Megat Abdul Wahab, Prof. Dr.
Keywords:	Dentin Root Resorption Enzyme-Linked Immunosorbent Assay Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	3-Jun-2020
Description:	The use of dentine sialophosphoprotein (DSPP) in the gingival crevicular fluid (GCF) has been suggested as a potentially safer and more sensitive alternative to the periodic radiographic method in diagnosing orthodontically induced inflammatory root resorption (OIIRR). This study aims to investigate the potential of DSPP in quantifying the severity of root resorption and to determine the effectiveness of the absorption spectroscopy method in the identification and quantification of DSPP compared with the Enzyme-Linked Immunosorbent Assay (ELISA) method. This cross-sectional study consisted of 45 subjects which were divided into 3 groups based on radiographic assessment on the level of root resorption; normal (R0), mild (RM) and severe (RS). DSPP in GCF samples was analysed using two techniques, the ELISA method and absorption spectroscopy method. In this study, the increase in the concentration level of DSPP coincided with the amount of root resorption which was evident in the existing radiographs. The DSPP concentration level measured by both techniques was the highest in the RS group (6.85+1.84 ng/ml) followed by the RM group (3.15+0.86 ng/ml) and the R0 group (2.22+0.84 ng/ml) with the lowest amount of DSPP. The increase was statistically significant in the RS group when compared to the R0 and RM group (p<0.05). There was increased of DSPP concentration between RM and R0, however, the difference in the means of DSPP concentration level between the R0 and RM group was insignificant (p>0.05). No statistically significant difference in mean concentration of DSPP was noted between ELISA and absorption spectroscopy method (p>0.05). This study showed that DSPP can be a potential marker of root resorption especially at severe levels. The fast and convenient operation of absorption spectroscopy method can favour it to be an alternative analytical method in the quantification of DSPP in GCF of patients with various severity of root resorption compared to ELISA method,Ijazah Sarjana Doktor Pergigian Klinikal (Ortodontik)
Pages:	101
Call Number:	WU220.T261h 2020 9 tesis
Publisher:	UKM, Kuala Lumpur
Appears in Collections:	Faculty of Dentistry / Fakulti Pergigian

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