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https://ptsldigital.ukm.my/jspui/handle/123456789/456129| Title: | Development and characterisation of plga nanoparticles containing xylitol as biofilms targeting wound delivery system |
| Authors: | Anam Anjum (P82806) |
| Supervisor: | Ng Shiow Fern, Associate Professor Dr. |
| Keywords: | Biofilms Microorganism Antibiotic Dissertations, Academic -- Malaysia |
| Issue Date: | 30-Oct-2017 |
| Description: | Biofilms are a sessile communities of microorganism enclosed in a self-produced extracellular polymeric matrix (EPS), which inhibits significant resistance towards antibiotic treatments. Recent studies have shown that xylitol possessed antibiofilm activity; however targeting drug delivery of xylitol to wound infections is still need to be investigated. The present investigation involved the synthesis of PLGA (polylactic co-glycolic acid) nanoparticles (NPs) incorporated with xylitol as a functional antibiofilm treatment. Preformulation studies of NPs were designed with different preparation techniques (solvent evaporation and nanoprecipitation), effect of process (homogenization and sonication) and formulation (surfactant and polymer concentration), then characterised for physiochemical properties and in vitro drug release. Next, NPs with the most desired physiochemical properties were incorporated with different xylitol concentrations (0.5%-5%) and re characterised. NPs displayed spherical morphology with mean particle size <200 nm and zeta potential -34.05 ± 3.68 mV. The in vitro drug release study showed overall release of 91.4 ± 0.5% with an average entrapment efficiency of 77.8 ± 1.7% and drug loading of 10.6 ± 4.6%. These NPs were then placed in bacterial cell cultures. Further, the results of crystal violet (CV) and XTT antibiofilm study showed the highest % of biofilm inhibition by NpX6 NPs. CV assay for S. aureus, P. aeruginosa and polymicrobial cultures were 77.46 ± 64%, 69.5 ± 12.1 %, and 79.3 ± 9.1% and XTT assay for S. aureus, P. aeruginosa and polymicrobial were 69.15 ± 11.67%, 63.4 ± 10.6 % and 63.7 ± 10.8 % respectively. The NPs penetration study was accomplished by a confocal laser scanning microscopy (CLSM) binded with Concanavaline A-fluorescein isothiocyanate (FITC). NPs produced fluorescence with increased intensity from 5 min to 30 mins due to the better NPs penetration into biofilm. LIVE/DEAD BacLight™ assay was also carried out to assess the bacterial viability. The Live/Dead viability assay images confirmed that NpX6 NPs have far better antibiofilm activity as compare to xylitol solution. NpX6 NPs were then incorporated into NaCMC hydrogel and again subjected to antibiofilm testings. The results indicated that hydrogel facilitated the release of xylitol and showed better biofilm inhibition. In summary, NpX6 NPs showed a high potential in overcoming biofilm associated wound infections.,Sarjana Sains |
| Pages: | 175 |
| Call Number: | QV20.5.A533d 2018 9 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Pharmacy / Fakulti Farmasi |
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|---|---|---|---|---|
| ukmvital_99885+Source01+Source010.PDF Restricted Access | 481.82 kB | Adobe PDF |  View/Open |
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