Lactatae dehydrogenase, tartrate resistant acid phosphatase and alkaline phosphatase activities in saliva during teeth movement

Abstract

During orthodontic treatment, progress of tooth movements can be classified into four stages, i.e. activation (related to tissue inflammation or cells' death), resorption, reversal and restructuring of new bones. Tooth movement is related to metabolic changes around the mouth. Therefore, the objective of this study is to determine enzymes profiles from patient's saliva during orthodontic tooth movement, i.e., lactate dehydrogenase (LDR) for inflammation, tartrate resistant acid phosphatase (TRAP) for bone resorption and alkaline phosphatase (ALP) for bone formation. Saliva samples were collectedfrom 6 orthodontic patients, 3 patients treated with extraction of premolar teeth and 3 with no extraction. Activities of all enzymes were measured before placement of orthodontic fixed appliances (normal activity) followed with day 3, 7, 10, 14, 17, 21, 24, 28 and 31 after the braces were activated. Result showed that for extraction and non-extraction treatment, all enzymes (LDH, TRAP and ALP) showed higher specific activities compared to normal activity. However, statistical analysis using "repeat measure" analysis showed that there were no significant (p>0.05) different between LDH, TRAP ALP specific activity in patients with extraction compared to patients with non-extraction. Meanwhile LDH showed significantly (p<0.05) increment at the early phase of treatment (Day 3,7 and 10), TRAP at day 14 and 17 followed by ALP at day 17, 21 and 24. As a cone/us ion, there were differences in profiles of enzymes activities as inflammation occurs during early phase of treatmentfollowed by bone resorption and boneformation.