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https://ptsldigital.ukm.my/jspui/handle/123456789/578816| Title: | Supplementation of antioxidant bht to different bull semen extenders enhances semen quality after chilling |
| Authors: | Khumran A. M (UPM) Yimer N (UPM) Rosnina Y (UPM) Ariff M. O (UPM) Wahid H (UPM) Kaka A (UPM) Ebrahimi M (UPM) Aliyu A. B (UPM) |
| Keywords: | Antioxidant Bull Butylated hydroxytoluene Extender Oxidation semen |
| Issue Date: | Feb-2017 |
| Description: | The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentrations of 0.5 in BX and 1.0 mM/mL in TEY and CEY compared to the controls. The BHT also showed a potential positive effect on progressive motility at 0.5 mM/mL in BX and 1.0 mM/mL in TEY and CEY. High concentrations of BHT (2.0 and 3.0 mM/mL), however, produced deteriorative effects on the sperm parameters in all the extenders. In conclusion, BHT when added at 0.5 mM/mL in BX and 1.0 mM/mL concentration to TEY and CEY extenders improved the quality parameters of bull chilled semen. |
| News Source: | Pertanika Journals |
| ISSN: | 1511-3701 |
| Volume: | 40 |
| Pages: | 131-142 |
| Publisher: | Universiti Putra Malaysia Press |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_116240+Source01+Source010.PDF | 262.47 kB | Adobe PDF |  View/Open |
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