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Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578816
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dc.contributor.authorKhumran A. M (UPM)
dc.contributor.authorYimer N (UPM)
dc.contributor.authorRosnina Y (UPM)
dc.contributor.authorAriff M. O (UPM)
dc.contributor.authorWahid H (UPM)
dc.contributor.authorKaka A (UPM)
dc.contributor.authorEbrahimi M (UPM)
dc.contributor.authorAliyu A. B (UPM)
dc.date.accessioned2023-11-06T03:08:16Z-
dc.date.available2023-11-06T03:08:16Z-
dc.date.issued2017-02
dc.identifier.issn1511-3701
dc.identifier.otherukmvital:116240
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578816-
dc.descriptionThe effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentrations of 0.5 in BX and 1.0 mM/mL in TEY and CEY compared to the controls. The BHT also showed a potential positive effect on progressive motility at 0.5 mM/mL in BX and 1.0 mM/mL in TEY and CEY. High concentrations of BHT (2.0 and 3.0 mM/mL), however, produced deteriorative effects on the sperm parameters in all the extenders. In conclusion, BHT when added at 0.5 mM/mL in BX and 1.0 mM/mL concentration to TEY and CEY extenders improved the quality parameters of bull chilled semen.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=1&journal=JTAS-40-1-2
dc.rightsUKM
dc.subjectAntioxidant
dc.subjectBull
dc.subjectButylated hydroxytoluene
dc.subjectExtender
dc.subjectOxidation
dc.subjectsemen
dc.titleSupplementation of antioxidant bht to different bull semen extenders enhances semen quality after chilling
dc.typeJournal Article
dc.format.volume40
dc.format.pages131-142
dc.format.issue1
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