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https://ptsldigital.ukm.my/jspui/handle/123456789/578668| Title: | Addition of l-tyrosine to improve betalain production in red Pitaya Callus |
| Authors: | Fadzliana N. A. F (UPM) Rogayah S (UPM) Shaharuddin N. A (UPM) Janna O. A (UPM) |
| Keywords: | Hylocereus polyrhizus Betalains Precursor L-tyrosine Pitaya callus Antioxidant |
| Issue Date: | Nov-2017 |
| Description: | The aim of this study is to enhance betalain production in red pitaya (Hylocereus polyrhizus) callus through supplementation of the precursor L-tyrosine. Red-pigmented calli were produced on Murashige and Skoog (MS) basal salt medium supplemented with 2 mg/L naphthaleneacetic acid (NAA), 4 mg/L thidiazuron (TDZ) and different concentrations of L-tyrosine (20, 40, 60, 80, 100 mg/L). The addition of 20 mg/L L-tyrosine was able to increase betacyanin production by 1.5-fold compared to calli grown on Murashige and Skoog (MS) basal salt medium (MSO). The results also revealed that betalain production (calli cultured with 20 mg/L L-tyrosine) was higher than MS medium with 2 mg/L NAA and 4 mg/L TDZ (without L-tyrosine) up to 1.7-fold. Four compounds, namely betacyanin, betaxanthin and two additional compounds, phenolic and flavonoid compounds, were detected in the red-pigmented calli treated with 20 mg/L L-tyrosine that were not found in the fresh fruit sample. This indicates that the callus system has potential to produce betalain pigment and that red-pigmented calli could be an important antioxidant source. |
| News Source: | Pertanika Journals |
| ISSN: | 1511-3701 |
| Volume: | 40 |
| Pages: | 521-532 |
| Publisher: | Universiti Putra Malaysia Press |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_116114+Source01+Source010.PDF | 1.06 MB | Adobe PDF |  View/Open |
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