Please use this identifier to cite or link to this item:
https://ptsldigital.ukm.my/jspui/handle/123456789/578368| Title: | Assessment of the genetic variation of malaysian durian varieties using inter-simple sequence repeat markers and chloroplast dna sequences |
| Authors: | Ging Yang Siew (UPM) Wei Lun Ng (UPM) Muhammad Fadzly Salleh (UPM) Sheau Wei Tan (UPM) Huynh Ky Noorjahan Banu Mohammed Alitheen (UPM) Soon Guan Tan (UPM) Swee Keong Yeap (UPM) |
| Keywords: | Chloroplast DNA sequence DNA barcoding Durio zibethinus Genetic diversity Intersimple sequence repeat |
| Issue Date: | Feb-2018 |
| Description: | To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties. |
| News Source: | Pertanika Journals |
| ISSN: | 1511-3701 |
| Volume: | 41 |
| Pages: | 321-332 |
| Publisher: | Universiti Putra Malaysia Press |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_115873+Source01+Source010.PDF | 554.52 kB | Adobe PDF |  View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.