1. UKM Learning and Research Repository
  2. Journal Article / Artikel Jurnal
  3. Journal Content Pages/ Kandungan Halaman Jurnal
Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578368
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGing Yang Siew (UPM)
dc.contributor.authorWei Lun Ng (UPM)
dc.contributor.authorMuhammad Fadzly Salleh (UPM)
dc.contributor.authorSheau Wei Tan (UPM)
dc.contributor.authorHuynh Ky
dc.contributor.authorNoorjahan Banu Mohammed Alitheen (UPM)
dc.contributor.authorSoon Guan Tan (UPM)
dc.contributor.authorSwee Keong Yeap (UPM)
dc.date.accessioned2023-11-06T03:00:52Z-
dc.date.available2023-11-06T03:00:52Z-
dc.date.issued2018-02
dc.identifier.issn1511-3701
dc.identifier.otherukmvital:115873
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578368-
dc.descriptionTo date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=1&journal=JTAS-41-1-2
dc.rightsUKM
dc.subjectChloroplast DNA sequence
dc.subjectDNA barcoding
dc.subjectDurio zibethinus
dc.subjectGenetic diversity
dc.subjectIntersimple sequence repeat
dc.titleAssessment of the genetic variation of malaysian durian varieties using inter-simple sequence repeat markers and chloroplast dna sequences
dc.typeJournal Article
dc.format.volume41
dc.format.pages321-332
dc.format.issue1
Appears in Collections:Journal Content Pages/ Kandungan Halaman Jurnal

Files in This Item:
File Description SizeFormat 
ukmvital_115873+Source01+Source010.PDF554.52 kBAdobe PDFThumbnail
View/Open
Show simple item record Recommend this item


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.