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https://ptsldigital.ukm.my/jspui/handle/123456789/578274| Title: | Anti-inflammatory effects of high-density lipoprotein via regulation of nitric oxide synthase expression and NF-?B transcription in activated human endothelial cells |
| Authors: | Wan Norhasanah Wan Yusoff (UITM) Yung-An Chua (UITM) Gabriele Ruth Anisah Froemming (UNIMAS) Abdul Manaf Ali (UNISZA) Hapizah Nawawi (UITM) |
| Keywords: | eNOS NF-?B Endothelial cells HDL LDL oxidation Protein expression |
| Issue Date: | Aug-2017 |
| Description: | Oxidation of low-density lipoprotein (LDL) and activation of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) are critical for the inflammatory response for endothelial dysfunction. The objective of this study is to investigate the effects of various doses of HDL on: (a) LDL susceptibility to oxidation; (b) expression of eNOS; and (c) expression of NF-?B p50 and p65. Different concentrations of HDL were incubated in LDL. The reaction rates of LDL susceptibility to oxidation were obtained by kinetic modeling analysis. For determination of eNOS, NF-?B p50 and p65 expression, different HDL concentrations were incubated in lipo polysacharides (LPS)-stimulated human umbilical vein endothelial cell line for 16 hours. Protein was extracted and analysed by western blot and nuclear transcription factor, for example, Co-incubation of LDL with increasing HDL concentrations showed longer lag time and lower reaction rate in a dose-dependent manner compared to controls (p<0.05). The eNOS expression at higher HDL concentration was significantly increased when compared to controls (p<0.05). HDL significantly decreased the expression of NF-?B p65 but not that of NF-?B p50. HDL protects LDL from oxidation, up regulates eNOS expression and down regulates the expression of NF-?B p65. These in part contribute to the role of HDL in the prevention and retardation of atherogenesis and atherosclerosis-related complications. |
| News Source: | Pertanika Journals |
| ISSN: | 0128-7680 |
| Volume: | 25 |
| Pages: | 49-62 |
| Publisher: | Universiti Putra Malaysia Press |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_115794+Source01+Source010.PDF | 1.1 MB | Adobe PDF |  View/Open |
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