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Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578274
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dc.contributor.authorWan Norhasanah Wan Yusoff (UITM)
dc.contributor.authorYung-An Chua (UITM)
dc.contributor.authorGabriele Ruth Anisah Froemming (UNIMAS)
dc.contributor.authorAbdul Manaf Ali (UNISZA)
dc.contributor.authorHapizah Nawawi (UITM)
dc.date.accessioned2023-11-06T02:59:49Z-
dc.date.available2023-11-06T02:59:49Z-
dc.date.issued2017-08
dc.identifier.issn0128-7680
dc.identifier.otherukmvital:115794
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578274-
dc.descriptionOxidation of low-density lipoprotein (LDL) and activation of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) are critical for the inflammatory response for endothelial dysfunction. The objective of this study is to investigate the effects of various doses of HDL on: (a) LDL susceptibility to oxidation; (b) expression of eNOS; and (c) expression of NF-?B p50 and p65. Different concentrations of HDL were incubated in LDL. The reaction rates of LDL susceptibility to oxidation were obtained by kinetic modeling analysis. For determination of eNOS, NF-?B p50 and p65 expression, different HDL concentrations were incubated in lipo polysacharides (LPS)-stimulated human umbilical vein endothelial cell line for 16 hours. Protein was extracted and analysed by western blot and nuclear transcription factor, for example, Co-incubation of LDL with increasing HDL concentrations showed longer lag time and lower reaction rate in a dose-dependent manner compared to controls (p<0.05). The eNOS expression at higher HDL concentration was significantly increased when compared to controls (p<0.05). HDL significantly decreased the expression of NF-?B p65 but not that of NF-?B p50. HDL protects LDL from oxidation, up regulates eNOS expression and down regulates the expression of NF-?B p65. These in part contribute to the role of HDL in the prevention and retardation of atherogenesis and atherosclerosis-related complications.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=2&journal=JST-25-S-8
dc.rightsUKM
dc.subjecteNOS
dc.subjectNF-?B
dc.subjectEndothelial cells
dc.subjectHDL
dc.subjectLDL oxidation
dc.subjectProtein expression
dc.titleAnti-inflammatory effects of high-density lipoprotein via regulation of nitric oxide synthase expression and NF-?B transcription in activated human endothelial cells
dc.typeJournal Article
dc.format.volume25
dc.format.pages49-62
dc.format.issueSpecial Issue
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