Please use this identifier to cite or link to this item:
https://ptsldigital.ukm.my/jspui/handle/123456789/578093| Title: | A competitive ELISA for quantification of protein A in culture medium |
| Authors: | Ismail B. Ahmad (Universiti Kebangsaan Malaysia) |
| Keywords: | Protein A Competitive ELISA IgG Staphylococcus aureus |
| Issue Date: | Dec-1996 |
| Description: | A competitive enzyme-linked immunosorbent assay (ELISA) for quantification of protein A produced by Staphylococcus aureus A676 (a methicillin-resistant strain) was based on competitive binding to rabbit IgG molecules between alkalinephosphatase-labelledprotein A and unlabelledprotein A. The optimum IgG concentration required for coating was 2-4 fig/ml. The optimum incubation time for colour development using (p-nitrophenol phosphate substrate was 20-30 min. The lowest protein A concentration that could be measured using the optimized competitive ELISA was 20 ng/ml, and the maximum 2 fig/ml. The amount of protein A produced in brain-heart infusion medium increased exponentially during log phase of cell growth, reaching a maximum concentration of 22.5 fig/ml after 15 h cultivation. |
| News Source: | Pertanika Journal of Tropical Agricultural Science |
| ISSN: | 0126-6128 |
| Volume: | 19 |
| Pages: | 95-102 |
| Publisher: | Universiti Putra Malaysia Press |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_131112+Source01+Source010.PDF | 1.33 MB | Adobe PDF |  View/Open |
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