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Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578093
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dc.contributor.authorIsmail B. Ahmad (Universiti Kebangsaan Malaysia)
dc.date.accessioned2023-11-06T02:58:24Z-
dc.date.available2023-11-06T02:58:24Z-
dc.date.issued1996-12
dc.identifier.issn0126-6128
dc.identifier.otherukmvital:131112
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578093-
dc.descriptionA competitive enzyme-linked immunosorbent assay (ELISA) for quantification of protein A produced by Staphylococcus aureus A676 (a methicillin-resistant strain) was based on competitive binding to rabbit IgG molecules between alkalinephosphatase-labelledprotein A and unlabelledprotein A. The optimum IgG concentration required for coating was 2-4 fig/ml. The optimum incubation time for colour development using (p-nitrophenol phosphate substrate was 20-30 min. The lowest protein A concentration that could be measured using the optimized competitive ELISA was 20 ng/ml, and the maximum 2 fig/ml. The amount of protein A produced in brain-heart infusion medium increased exponentially during log phase of cell growth, reaching a maximum concentration of 22.5 fig/ml after 15 h cultivation.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journal of Tropical Agricultural Science
dc.relation.urihttp://www.pertanika.upm.edu.my/view_archives.php?journal=JTAS-19-2%263-12
dc.rightsUniversiti Putra Malaysia Press
dc.subjectProtein A
dc.subjectCompetitive ELISA
dc.subjectIgG
dc.subjectStaphylococcus aureus
dc.titleA competitive ELISA for quantification of protein A in culture medium
dc.typeJournal Article
dc.format.volume19
dc.format.pages95-102
dc.format.issue2-3
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