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https://ptsldigital.ukm.my/jspui/handle/123456789/576804| Title: | Characterisation of pathogenesis-related genes and resistance gene candidates in banana (musa acuminata) and their expression during host-pathogen interaction |
| Authors: | Chee-Yong Yang (UPM) Sathyapriya H (UPM) Mui-Yun Wong (UPM) |
| Keywords: | Banana Fusarium wilt Nucleotide-binding site PR proteins resistance-gene candidate Serine/ threonine kinase |
| Description: | Amplified chitinase gene sequence shared 99% homology with Musa acuminata class III acidic chitinase and beta-1,3-glucanase gene sequence was 100% homologous to Musa x paradisiaca beta-1,3-glucanase. Three nucleotide-binding sites and the leucine-rich repeat (NBS-LRR) type of putative RGCs and one serine/threonine kinase gene were characterised at the amino acid level. Kinase-2 (LVLDDVW) and kinase-3 (GSRIIITTRD) motifs in the nucleotide-binding domain were highly conserved in RGC2 and RGC3 and these genes belong to the non-TIR-NBS class RGCs. RGC1 was also clustered into non-TIR-NBS class RGCs; however, many residue substitutions were present in the kinase-2 and kinase-3 motifs. The sub-domain IX (LTEKSDVYSFGVVL) of serine/threonine protein kinase was highly conserved in RGC5 and it shared highest homology with PTH-2 from muskmelon. RT-PCR analysis revealed the differential expression of PR and RGC genes exhibited by different banana genotypes over sampling time. Chitinase was expressed during banana-FocR4 interaction in all three banana genotypes. However, its expression was high and constant in ‘Rastali Mutiara’ during banana-FocR4 interaction and resulted in very low disease severity in FocR4 inoculated plants (2%) compared to ‘Rastali wild-type (16%) and ‘Jari Buaya’ (8%) at six weeks after inoculation. This suggests that chitinase may play an important role in disease resistance against FocR4. Besides, our study also shows that ‘Rastali Mutiara’ can be a potential source of disease-resistant genes for molecular breeding of banana. |
| News Source: | Pertanika Journals |
| ISSN: | 1511-3701 |
| Volume: | 39 |
| Pages: | 55-72 |
| Publisher: | Universiti Putra Malaysia |
| URI: | https://ptsldigital.ukm.my/jspui/handle/123456789/576804 |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_82440+Source01+Source010.PDF | 1.33 MB | Adobe PDF |  View/Open |
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