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Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/576804
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dc.contributor.authorChee-Yong Yang (UPM)
dc.contributor.authorSathyapriya H (UPM)
dc.contributor.authorMui-Yun Wong (UPM)
dc.date.accessioned2023-11-06T02:08:10Z-
dc.date.available2023-11-06T02:08:10Z-
dc.identifier.issn1511-3701
dc.identifier.otherukmvital:82440
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/576804-
dc.descriptionAmplified chitinase gene sequence shared 99% homology with Musa acuminata class III acidic chitinase and beta-1,3-glucanase gene sequence was 100% homologous to Musa x paradisiaca beta-1,3-glucanase. Three nucleotide-binding sites and the leucine-rich repeat (NBS-LRR) type of putative RGCs and one serine/threonine kinase gene were characterised at the amino acid level. Kinase-2 (LVLDDVW) and kinase-3 (GSRIIITTRD) motifs in the nucleotide-binding domain were highly conserved in RGC2 and RGC3 and these genes belong to the non-TIR-NBS class RGCs. RGC1 was also clustered into non-TIR-NBS class RGCs; however, many residue substitutions were present in the kinase-2 and kinase-3 motifs. The sub-domain IX (LTEKSDVYSFGVVL) of serine/threonine protein kinase was highly conserved in RGC5 and it shared highest homology with PTH-2 from muskmelon. RT-PCR analysis revealed the differential expression of PR and RGC genes exhibited by different banana genotypes over sampling time. Chitinase was expressed during banana-FocR4 interaction in all three banana genotypes. However, its expression was high and constant in ‘Rastali Mutiara’ during banana-FocR4 interaction and resulted in very low disease severity in FocR4 inoculated plants (2%) compared to ‘Rastali wild-type (16%) and ‘Jari Buaya’ (8%) at six weeks after inoculation. This suggests that chitinase may play an important role in disease resistance against FocR4. Besides, our study also shows that ‘Rastali Mutiara’ can be a potential source of disease-resistant genes for molecular breeding of banana.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=1&journal=JTAS-39-1-2
dc.subjectBanana
dc.subjectFusarium wilt
dc.subjectNucleotide-binding site
dc.subjectPR proteins
dc.subjectresistance-gene candidate
dc.subjectSerine/ threonine kinase
dc.titleCharacterisation of pathogenesis-related genes and resistance gene candidates in banana (musa acuminata) and their expression during host-pathogen interaction
dc.typeJournal Article
dc.format.volume39
dc.format.pages55-72
dc.format.issue1
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