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2. PHD Thesis / Tesis PHD
3. Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

Title:	Production of bio-succinic acid from oil palm fronds using Actinobacillus succinogenes ATCC 55618
Authors:	Tan Jian Ping (P71139)
Supervisor:	Jamaliah Md Jahim, Prof. Dr.
Keywords:	Oil palm Actinobacillus Biochemical engineering Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	22-May-2017
Description:	Bio-succinic acid had been commercialized since 2012 and the market volume was forecasted to bloom with an average of compound annual growth rate of 25% from 2015 until 2020 since the introduction of bio-production. Oil palm frond (OPF) in this regards, contributes to 70 % of the residue from palm oil industry and could potentially serves as the cheapest carbon source for the production of bio-succinic acid. This research attempts to evaluate the potential of abundant OPF that could be served as the feed carbon source for bio-succinic acid production. The characterization of OPF was done according to National Renewable Energy Laboratory protocols. The result of the characterization showed high carbohydrate content in OPF bagasse and high fermentable sugar content in OPF juice. The fermentability of raw OPF juice was carried out by Actinobacillus succinogenes in serum bottles and its ability to serve as the carbon source was proven. Bio-succinic acid yield was optimized by manipulating the culture medium in the serum bottle. Tryptone was found to be the best nitrogen source with 22.4 g/L of final succinate concentration. Magnesium Carbonate (MgCO3) was identified as a better carbonate for bio-succinic acid production with 0.64 g/g of final succinate yield. Optimum conditions for the highest bio-succinic acid production were determined. After the optimized medium is achieved, the effects of CO2 sparging rate and fermentation profile were investigated in a 3.5 L bench-top bioreactor. The higher sparging rate of CO2 promoted the CO2 transfer to the medium that led to higher bio-succinic acid production of 34.1 g/L. During bioreactor fermentation, the overall fermentation period was shortened and more sugar was utilized. The commercial potential and the feasibility of bio-succinic acid production from OPF in Malaysia were studied. Market supply and demand on bio-succinic acid were analysed from different market forecasting companies. Process flow diagram was designed using SUPERPRO designer. Mass balance, equipment design, equipment cost and profitability analysis were manually performed. The manual calculations were compared with the simulation performed using SUPERPRO designer. Economic analysis showed that bio-succinic acid production from OPF has a bright potential to be commercialized in Malaysia. The plant capacity volume was set at 65 MMT per annum by using 1 % of the overall OPF available in Malaysia, the Return on Investment (ROI) was calculated to be doubled the minimum acceptable ROI. Payback Period (PBP) was estimated to be shorter than the reference PBP. The net return is 26 million RM, which is a positive value. These show that the commercial bio-succinic acid plant in Malaysia is highly profitable and attractive.,Certification of Master's / Doctoral Thesis" is not available
Pages:	206
Call Number:	TP248.3.T254 2017 3 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

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