Phytotoxic activity of Tinospora Tuberculata leaf methanol extract on the growth and development of rice and associated weed species

Abstract

Tinospora Tuberculata is an aromatic shrub with pharmacological properties. We assessed T. Tuberculata for its ability to suppress growth of rice weeds. Utilization of leaf extract of T. Tuberculata as pre-emergence application inhibited the germination and growth of tested weed species and increased the yield of transplanted rice in the glasshouse condition. Ultra-Fast Liquid Chromatography (UFLC) analysis exhibited leaf extract contained high number and amount of chemical compounds. The results showed that 11 of the 32 components in the leave are allelochemicals, including benzoic acid, caffeic acid, Chlorogenic acid, Isoorientin, Isovitexin, Orientin, P-Anisic Acid, Syringic Acid, Trans-Cinnamic Acid, Trans-Ferulic Acid, and Vitexin have the same retention time with those peaks of the extract. The amount of compounds was present in the range of between 4817 and 115.5 mg kg-1 dry weight (DW). These results suggest that T. Tuberculata leaf contains a significant source of plant growth inhibitors with potential to use as a natural pre-emergence herbicide.