Cryosurvival of goramy (osphronemus goramy, Lacepede 1801) Spermatozoa after subzero freezing

Abstract

Combination eject of 1 0% of methanol either with 0.5% of sucrose, or 15% of skim milk on viability, motility, abnormality, and spermatozoa concentration of Osphronemus goramy, Lacepede 1801, 24 hour postcryopreservation has been studied. Hypophyzation (0.7 ml ovaprim/kg body weight) was conducted seven hours before stripping. Fresh semen were whitish color, pH 8, and 100 0 I of volume per ejaculate. 1he percentage of sperm viability, motility: abnormality, and spermatozoa concentration of fresh semen were: 86%, 72. 73%, 13%, and 22.75 x 107 cell/ml, respectively. The highest post-thawed sperm viability (36%) was the combination of 0.5% sucrose-Kurokura 11 extender and 10% methanol, while the highest post-thawed sperm motility (66.67%) was the combination of 15% skim milk-Fish ringer and 10% methanol. The lowest post-thawed sperm abnormality (30%) was the combination of 0.5% sucrose-Kurokura 11 extender and 10% methanol, while the highest post-thawed sperm concentration (4.88 x 107 cell/ml ejaculate) was the combination of 15% skim milk-Fish ringer and 10% methanol.