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https://ptsldigital.ukm.my/jspui/handle/123456789/519490| Title: | Design, Fabrication And Characterization Of A Microfluidic Device For Isolation Of Human White Blood Cells Using Elastomeric Polyurethane Methacrylate |
| Authors: | Jafar Alvankarian (P49096) |
| Supervisor: | Burhanuddin Yeop Majlis, Prof. Dato' Dr. |
| Keywords: | Design Fabrication And Characterization Of A Microfluidic Device A Microfluidic Device Design Fabrication And Characterization Isolation Of Human White Blood Cells Isolation Of Human White Blood Cells Using Elastomeric Polyurethane Methacrylate Elastomeric Polyurethane Methacrylate Microfluidic devices |
| Issue Date: | 15-Jan-2013 |
| Description: | The field of microfluidic devices for biomedical applications is growing very fast. These systems offer advantages over conventional lab methods in terms of low reagent/sample usage, short analysis time, high degree of automation/integration, and portability with a wide range of applications in biochemical, disease detection, and point-of-care analysis. The variety of physiochemical properties and low-cost fabrication techniques has diverted the attentions of the researchers from silicon-based to polymeric substrates for microfabrication. The objective of this research is to design, fabricate, and characterize a new pillar-based microfluidic device for sizebased separation of human blood cells on a new elastomeric substrate with application in the low-cost rapid prototyping of lab-chip devices. First, a UV-curing elastomeric Polyurethane Methacrylate (PUMA) is introduced and characterized for rapid prototyping of the device. Swelling and solubility of PUMA in different chemicals is determined. The water contact angle measurements show that the native PUMA is hydrophilic with water contact angle of 86 without surface treatment. The current monitoring method is used for measurement of the electro-osmotic flow mobility in the microchannels made from PUMA. Optical transparency, physical, thermal, and mechanical properties of PUMA are evaluated. UV-lithography and molding method is used for making micropillars and deep channels. A device is fabricated and tested for examining the strength of different bonding techniques such as conformal, corona discharge and semi-curing of two PUMA layers. Second, a single inlet single outlet device is introduced that uses parallel U-shape arrays of pillars with cutoff size of 5.5 μm for trapping WBCs in a pillar chamber with internal dead-volume of less than 1.0 μl. The microstructures are designed to limit the deformation against fluid pressures. Numerical analysis showed that at a maximum pressure loss of 15 kPa which is lower than the device conformal bonding strength of ~100 kPa, the pillar deformation is less than 5% for flow rates of up to 1.0 ml min-1. Molding technique was employed for device prototyping using PUMA resin and PDMS mold. Characterization of the duallayer device with beads and blood samples are performed. Tests with blood injection showed that ~18-25% of WBCs are trapped and ~84-89% of RBCs are passed at flow rates of 15-50 μl min-1 with a slight decrease of WBCs trap and improve of the RBCs pass at higher flow rates. Similar results were obtained by separation of mixed microspheres of different size injected at flow rates of up to 400 μl min-1. Tests with blood samples stained by fluorescent gel demonstrated that the WBCs are accumulated in the arrays of pillars that later ends up to blockage of the device. Filtration results of using elastomeric substrate present a good consistency with the trend of separation efficiencies of the similar silicon-based filters.,PhD |
| Pages: | 170 |
| Call Number: | TJ853.4.M53 .A456 2013 3 |
| Publisher: | UKM, Bangi |
| Appears in Collections: | Institute of Microengineering and Nanoelectronics / Institut Kejuruteraan Mikro dan Nanoelektronik (IMEN) |
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