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Title:	Transcriptomic and metabolomic profiling of polygonum minus leaves upon methyl jasmonate elicitation
Authors:	Tajadod Reyhaneh Rahnamaie (P60034)
Supervisor:	Normah Mohd Noor, Pof.Dr.
Keywords:	Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia Polygonum Botany
Issue Date:	25-Jul-2017
Description:	Polygonum minus Huds. (kesum) is an aromatic plant that grows in Southeast Asia and traditionally used as medicine. This plant produces diverse secondary metabolites, such as terpenoids, phenylpropanoids and their derivatives, which are known to play a role in plant response against environmental stresses. Methyl jasmonate (MeJA) is a plant signaling molecule commonly applied to elicit biotic stress responses. However, the effect of MeJA in inducing the accumulation of secondary metabolites in P. minus is not well studied due to limited genetic information. In this study, the effect of exogenous MeJA treatment on the composition of volatile organic compounds (VOCs) and genome-wide expression profile in the leaf tissue of P. minus was respectively investigated through metabolomic and transcriptomic approaches. Timecourse changes in the VOC compositions of P. minus leaves on day 1, 3 and 5 upon MeJA treatment were analyzed through solid-phase microextraction (SPME) following gas chromatography-mass spectrometry (GC-MS) followed by multivariate data analysis. The highest accumulation of secondary metabolites was observed at 24 hours after MeJA treatment. Therefore, RNA-seq analysis on P. minus leaf samples of 24 h after treatment using short read sequencing technology Illumina HiSeq™ 2000 system was employed to profile the leaf transcriptome and then to identify differentially expressed genes (DEGs). A total of 182,111 unique transcripts (UTs) were obtained by de novo assembly of 191.57 million paired-end clean reads through Trinity analysis pipeline. According to the fold changes, p-value and FDR parameters, a total of 2,374 UTs were identified to be significantly up-/down-regulated 24 hours after MeJA treatment. These UTs comprise many genes related to plant secondary metabolite biosynthesis, defense and stress responses. RT-qPCR analysis was performed to validate the expression of 27 candidate genes. MeJA elicitation is found to up-regulate endogenous JA biosynthesis and also most of the key genes in JA signaling and biosynthetic pathways determined, which indicating the presence of JA signaling pathway in P. minus leaves. MeJA also triggered the upregulation of phenylpropanoid pathway. Through the integrated transcriptomic and metabolomics approaches, this study sheds light on the molecular mechanism underlying physiological responses of P. minus to MeJA elicitation and assists us to better understand how transcriptional regulation related to the biosynthesis of secondary metabolites is mediated by the exogenous application of MeJA.,Certification of Masters/ Doctorial Thesis" is not available
Pages:	172
Call Number:	QK495.P78T334 2017 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Institute of Systems Biology / Institut Biologi Sistem (INBIOSIS)

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