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https://ptsldigital.ukm.my/jspui/handle/123456789/515375Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Wan Mohd Aizat Wan Kamaruddin, Assoc. Prof. Dr. | |
| dc.contributor.author | Ili Nadhirah Jamil (P94816) | |
| dc.date.accessioned | 2023-10-16T08:36:14Z | - |
| dc.date.available | 2023-10-16T08:36:14Z | - |
| dc.date.issued | 2022-03-17 | |
| dc.identifier.other | ukmvital:129820 | |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/515375 | - |
| dc.description | Garcinia mangostana L., commonly known as mangosteen, contains beneficial bioactive compounds such as xanthones that exhibit high antioxidant, anti-inflammatory, and anti-cancer activities. Previously, various omics platforms such as transcriptomics and metabolomics were used to elucidate the complex ripening process of mangosteen. However, the molecular regulation of mangosteen ripening and metabolic alterations have not been thoroughly understood, particularly at the proteome level. This is the first proteomics study on mangosteen ripening that uses state-of-the-art Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) analysis whereby the instrument allows more scanning of peptide fragments, thus increasing proteome coverage compared to other mass spectrometry instrument. Additionally, postharvest analyses such as color changes, anthocyanin content, antioxidant activity, soluble solid content, and titratable acidity were conducted to confirm the proteome alterations. A total of 176 out of 1618 proteins such as 1-aminocyclopropane-1-carboxylate oxidase (ACO) (ethylene biosynthesis), pyruvate kinase (PK) (carbohydrate metabolism), polygalacturonase (PG) (cell wall modification), phenylalanine lyase (PAL) (secondary metabolite biosynthesis), and 2,4,6-trihydroxybenzophenone synthase (BPS) (xanthone biosynthesis) were statistically classified as differentially expressed proteins (DEPs). Integration of proteomics datasets with previous omics study (transcriptomics and metabolomics) using integration tools (MapMan and Paintomics3) further revealed weak correlations between omics possibly due to post-transcriptional and post-translational regulations. Pathway mapping highlights enzymes such as ACO, PAL, and BPS were regulated at both transcript and protein levels, thus promoting ethylene, anthocyanin, and xanthone accumulations during ripening. This study unravels the key molecular components responsible for ripening related changes in this fruit which will be beneficial for developing strategies to prolong its shelf-life. The molecular insights from these multiomics studies further shed some lights onto biochemical regulation of the xanthone, yet such variegated and complex data can be highly inorganized for research use without a dedicated database. Xanthone is the focus of this study because its biosynthetic pathway is yet to be fully characterized, compared to other known phenolics. This study serves as one of the pioneering systems biology efforts to unravel the xanthone pathway in plants which could be highly diverse among species by constructing a Xanthone Database (XDb) was that serves as a central information hub to retrieve information about molecular expression as well as other mangosteen-related information (e.g., postharvest), which may benefit potential researchers in mangosteen or xanthone area, as well as other users.,Ph.D | |
| dc.language.iso | eng | |
| dc.publisher | UKM, Bangi | |
| dc.relation | Institute of Systems Biology / Institut Biologi Sistem (INBIOSIS) | |
| dc.rights | UKM | |
| dc.subject | Mangrove plants | |
| dc.subject | Mangrove ecology | |
| dc.subject | Universiti Kebangsaan Malaysia -- Dissertations | |
| dc.subject | Dissertations, Academic -- Malaysia | |
| dc.title | Multiomics integration of mangosteen pericarp during ripening and xanthone database development | |
| dc.type | Theses | |
| dc.format.pages | 131 | |
| dc.identifier.callno | QK938.M27I445 2022 tesis | |
| dc.identifier.barcode | 006695(2022) | |
| Appears in Collections: | Institute of Systems Biology / Institut Biologi Sistem (INBIOSIS) | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_129820+SOURCE1+SOURCE1.0.PDF Restricted Access | 3.84 MB | Adobe PDF |  View/Open |
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