Please use this identifier to cite or link to this item:
https://ptsldigital.ukm.my/jspui/handle/123456789/515020Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Burhanuddin Yeop Majlis, Prof. Dr. | |
| dc.contributor.author | Alireza Bahadorimehr (P48937) | |
| dc.date.accessioned | 2023-10-16T07:53:47Z | - |
| dc.date.available | 2023-10-16T07:53:47Z | - |
| dc.date.issued | 2012-05-24 | |
| dc.identifier.other | ukmvital:120123 | |
| dc.identifier.uri | https://ptsldigital.ukm.myjspui/handle/123456789/515020 | - |
| dc.description | Microfluidic systems have become increasingly well-known in different fields of studies. In recent years, research and development on lab-on-a-chip (LOC) and micro total analysis systems (μ-TAS) have been rapidly growing. Microfluidic devices are becoming one of the most dynamic parts of BioMEMS technology. The main applications of microfluidics are medical diagnostics, genetic sequencing, chemistry, drug delivery, and proteomics. Bioseparation techniques that are widely used in biotechnology become more difficult as the quantity of the materials to be purified are very small, especially, in biological cell separation from suspensions with high fragility and aggregation possibilities. The use of magnetic field for separation of small magnetic particles is a well-known technique in biology. The magnetic fields can manipulate biological cells tagged by magnetic particles that are suspended inside the microfluidic system. One of the most important strength of this technique is that it provides probably the most convenient and rapid method in separating desired particles from diluted solutions because magnetic fields are transparent to the cells and leave them without any damage. In biology, separation of specific target protein from a biological sample is very important in order to detect small amounts of antigens and biomolecules. For this purpose magnetic micro-particles have been used to separate and manipulate biomolecules. In this method magnetic beads are attached to target cells using specific ligands. This work describes a microfluidic device that can separate magnetic particles from suspended liquid by activating a planar microcoil in order to generate magnetic force on micro magnetic particles. Injection of magnetic beads and ferrofluids through the inlet channel transfers them to the main chamber in which magnetic field is applied. The magnetic field generated by the planar spiral coil instead of using permanent magnet yields several advantages in design flexibility and compactness. The fabrication of the microelectromagnet coil is based on a microscale winding process combined with nickel electroplating as a magnetic core to enhance the forces on magnetic particles at separation chamber. Two thin layers of polydimethylsiloxane (PDMS) and SU-8 are used to decrease the distance between microcoil and microchannel. Electromagnetic and thermoelectric simulation is performed in order to calculate the magnetic field and temperature distribution in the system. The bead capturing behavior is demonstrated in the chamber using three types of magnetic particles with 200 nm, 750 nm and 2.8 μm diameters. The experimental results show optimum capturing efficiency with dc current of 1 A passing through the microcoil. At this current, maximum magnetic flux density of 0.022 Tesla is achieved in the fluid microchannel, and the magnetic microparticles in buffer solution moved toward the bottom of the fluidic chamber where the coil is located. The maximum magnetic flux density gradient of 110 (T/m) is obtained at the center of the microchamber. When the dc current is removed the particles release easily. The described microelectromagnetic part for the first time features the ability to separate from the microfluidic part in order to use in other fluidic designs.,Certification of Master's / Doctoral Thesis" is not available | |
| dc.language.iso | eng | |
| dc.publisher | UKM, Bangi | |
| dc.relation | Institut Kejuruteraan Mikro dan Nanoelektronik (IMEN) / Institute of Microengineering and Nanoelectronics | |
| dc.rights | UKM | |
| dc.subject | Fluid mechanics | |
| dc.subject | Biomolecules -- Analysis | |
| dc.subject | Biotechnology -- Materials | |
| dc.subject | Microfluidics | |
| dc.subject | Universiti Kebangsaan Malaysia -- Dissertations | |
| dc.subject | Dissertations, Academic -- Malaysia | |
| dc.title | Magnetic microparticle separation in lab on a chip system for biomolecule detection applications | |
| dc.type | Theses | |
| dc.format.pages | 266 | |
| dc.identifier.callno | TJ853.4.M53B337 2012 3 tesis | |
| dc.identifier.barcode | 004064(2019) | |
| Appears in Collections: | Institute of Microengineering and Nanoelectronics / Institut Kejuruteraan Mikro dan Nanoelektronik (IMEN) | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_120123+SOURCE1+SOURCE1.0.PDF Restricted Access | 782.37 kB | Adobe PDF |  View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.