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Title:	Production of biocellulose by gluconobacter oxydans strain pin7 using date fruit waste in agitated fermentation
Authors:	Alabbosh Khulood Fahad S. (P78386)
Supervisor:	Nur Hazlin Hazrin Chong, Dr.
Keywords:	Microbial biotechnology Cellulose Gluconobacter oxydans Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	29-Apr-2021
Description:	Bacterial cellulose (BC) is an important current and future organic compound due to its application in many different industries such as food, pharmaceutical, textile and biomedical industries. However, low BC concentration and productivity, in addition to the expensive cost of raw materials, limit its production. Currently, there is a significant production of date fruit waste (Phoenix dactylifera) in the Kingdom of Saudi Arabia (KSA) that contributed to a major economic loss in the country. Therefore, this study aims to explore the usage of date fruit waste from KSA as a carbon source for BC production using a locally isolated BC-producing bacterium. BC-producing bacteria were screened from rotten fruits obtained from local supermarkets through batch fermentation in the medium of Hestrin-Schramm (HS). The bacterial strain producing the highest BC value (3.34 ± 0.06 g/L and 0.49 g/L/day) from pineapple was molecularly identified by 16S rRNA sequencing as Gluconobacter oxydans PIN7. Through X-ray Powder Diffraction (XRD), it was shown that the cellulose material produced by PIN7 had high crystallinity (71.4%) and high alpha-cellulose content (73%), characteristic of cellulose from bacteria. PIN7 was selected for further growth optimisation by using date fruit waste as the sole carbon source. For this purpose, five varieties of rotten dates (Sukari, Fankha, Magholah, Hamra and Gaspah) were tested as potential carbon sources for G. oxydans in agitated fermentation to produce the highest amount of BC compared to HS. BC production using Sukari as the sole carbon source resulted in 11.79 ± 0.75 g/L of BC produced, which was the highest amongst all the date types tested and 252.99% higher than HS medium.Further media optimisation was performed by testing HS media components (i.e. peptone, yeast extract, Na2HPO4 and citric acid) singularly with Sukari syrup and analysing subsequent BC production by PIN7. Peptone (9 g/L) with Sukari syrup (100 g/L) was found to produce the highest yield of BC (16.56 ± 0.16 g/L) and productivity (2.37 ± 0.02 g/L/day). Finally, Response Surface Methodology (RSM) was used to determine the optimum temperature, pH, and shaker speed against cellulose yield and it was found that BC production by PIN7 was the greatest at 27.50 °C, pH 6, and 180 rpm, yielding 22 g/L and productivity of 3.14 g/L/day. This resulted in an increase of 558.7% compared to the original procedure (i.e. using HS medium without optimisation). Further, BC characterisation using Fourier-transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy (FESEM) showed there was no significant difference in BC structure after optimisation. This study successfully demonstrated high BC production from a locally isolated strain using the date fruit waste as the sole carbon source. Compared to the current BC production processes, outcomes from this study provide a more sustainable, low-cost and high-yield and productivity alternative for various industrial appplications.,Ph.D
Pages:	161
Call Number:	TP248.27.M53.A433 2021 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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