Optimisation and production of alpha amylase from thermophilic Bacillus spp. in degradation of food waste

Marwan Jawad Kadhim Msarah (P84491)

Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/500257

Full metadata record

DC Field	Value	Language
dc.contributor.advisor	Wan Syaidatul Aqma Wan Mohd Noor, Dr.	-
dc.contributor.author	Marwan Jawad Kadhim Msarah (P84491)	-
dc.date.accessioned	2023-10-13T09:40:33Z	-
dc.date.available	2023-10-13T09:40:33Z	-
dc.date.issued	2019-09-24	-
dc.identifier.other	ukmvital:112354	-
dc.identifier.uri	https://ptsldigital.ukm.my/jspui/handle/123456789/500257	-
dc.description	Thermophilic bacteria received global attention due to their biotechnological importance as they possess unique enzymes with high activity and stability at elevated temperatures. This work aimed to optimise α-amylase production by thermophilic Bacillus sp. in order to degrade food waste. A total of 23 thermophilic strains were isolated from 9th May until 14th July 2016 Dusun Tua, Selangor and Sungai Klah, Perak Hot Springs in Malaysia. These isolates were confirmed as thermophiles by growing them on Thermus agar medium (ATCC medium 697), screened for six extracellular enzyme activities and biochemical tests according to Bergey's manual of determinative bacteriology. Two strains HULUB1 and SUNGB2 with high production of α-amylase were selected for further studies and characterised by API 50CHB and 16S rRNA sequencing. Enzyme α-amylase was extracted and partially purified to 80% using ammonium phosphate, then characterised for pH and temperature profiles. Further optimisation was carried out by applying the response surface methodology (RSM) with a central composite design (CCD). Furthermore, the two isolates and the α-amylase extracted were used separately in degradation of food waste. Results showed that the strains identified as Bacillus licheniformis HULUB1 and Bacillus subtilis SUNGB2 were Gram (+), aerobic endospore-forming rods, can grow on 12% NaCl concentration and up to 55°C. The strains showed amylolytic and cellulolytic activities, being capable of utilising various carbon sources (API50 CHB) and produce α-amylase. Further, using RSM the optimum conditions for the production of α-amylase were found to be at pH 6 and temperature 65°C, giving amylase activity of 26.65 U/mL for HULUB1 and 32.84 U/mL for SUNGB2. Stability of α-amylase with significant levels of enzyme activity could be recorded at 55-85°C and pH 5.0-9.0 ranges. The specific activity obtained for HULUB1 were increased 10 fold (10.66 U/μg) and 21 fold (5.40 U/μg) increased for SUNGB2 compared to the crude enzymes. The food waste treatment showed the greatest reduction was achieved with the mixed culture of HULUB1 and SUNGB2 isolates at day 12 with 43% solid content at 45°C. Meanwhile, the degradation with amylase treatment by a mixed amylase HULUB1 and SUNGB2 were achieved at day 12 and 65°C with 45% solid content. Moreover, enzymatic products of these isolates are worth further attention due to their potential contribution to food waste treatment. Certification of Master's/Doctoral Thesis is not available,Ph.D.	-
dc.language.iso	eng	-
dc.publisher	UKM, Bangi	-
dc.relation	Faculty of Science and Technology / Fakulti Sains dan Teknologi	-
dc.rights	UKM	-
dc.subject	Thermophilic strains	-
dc.subject	Bacillus spp.	-
dc.subject	Food waste	-
dc.subject	Universiti Kebangsaan Malaysia -- Dissertations	-
dc.subject	Dissertations, Academic -- Malaysia	-
dc.title	Optimisation and production of alpha amylase from thermophilic Bacillus spp. in degradation of food waste	-
dc.type	Theses	-
dc.format.pages	215	-
dc.identifier.barcode	004249(2019)	-
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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