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https://ptsldigital.ukm.my/jspui/handle/123456789/486901Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | ZahiraYaakob, Prof. Ir. Dr. | - |
| dc.contributor.author | Md. Moniruzzaman (P78415) | - |
| dc.date.accessioned | 2023-10-11T02:26:23Z | - |
| dc.date.available | 2023-10-11T02:26:23Z | - |
| dc.date.issued | 2018-04-23 | - |
| dc.identifier.other | ukmvital:100868 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/486901 | - |
| dc.description | Common fig (Ficus carica L.) is an economical important crop because of the taste, nutrition and medicinal values of its fruit. Asexual propagation (cutting, air layering, grafting) is usual for the plant as it doesn't produce viable seeds for germination. Poor survival rate, season dependency, intensive labor, prolong time and sacrifice of mother plant are the major drawbacks of conventional fig propagation methods. In Malaysia, fig has been recognized as an emerging future crop. However, appropriate cultivar selection, availability of good quality propagation materials of elite genotypes and pest susceptibility are the major limitations. This study was carried out to select well performing fig cultivars, to develop an in vitro regeneration method and to determine the genetic stability of regenerated plants. Seventeen fig cultivars of different origins were collected, propagated and maintained in a greenhouse. Data of 15 parameters on plant growth, fruit yield, fruit characteristics and pest (mealybug) infestation were recorded and analyzed to select well performing cultivars for future study. Shoots from three cultivars namely- Masui Dauphine, Orphan and A134 were selected for in vitro regeneration. Myzim SC (fungicide), NaOCl (sodium hypochlorite) and HgCl2 (mercuric chloride) were used for surface sterilization of shoot explants. Murashige and Skoog (MS) media supplemented with 6-Benzylaminopurine (BAP), 1-Naphtthaleneacetic Acid (NAA) and Giberellic Acid (GA3) were used to develop media for shoot induction and proliferation, shoot elongation and rooting. Effect of media modification (half calcium, half ammonium nitrate, half sugar) and culture system (liquid and solid media) were studied for media optimization. To determine the genetic stability, DNA was extracted from fresh leaf of donor and regenerated plants. Thirteen ISSR (Inter Simple Sequence Repeats) primers were screened to evaluate the banding pattern and band position. The study showed that 10 cultivars out of 17 performed well in Malaysian climate. The selected cultivars are Orphan, A134, A132, Dauphine, Masui Dauphine, B110, Longue d'Aout, Wuhan, Alma and Fen Chan Huang. Masui Dauphine produced the heaviest fruit (77.7 gm/fruit). Meanwhile, Fen Chan Huang produced the maximum number of fruits (119) per plant with fruit of 24.8 gm/fruit. The maximum yield of fruit (3410 gm/per plant) was produced by the cultivar A134. The fruit brix for Alma was approximately 24 %, which was higher than the average fruit brix for other selected cultivars (20 %). The best surface sterilization response for in vitro regeneration was found by the treatment of 100 µl/ml Myzim SC for 15 min followed by 5 % NaOCl for 4 min and finally 0.1 % HgCl2 for 1 min which gave approximately 38 % shoots sterility. Half calcium modified MS (HCMS) liquid media supplemented with 100 mg/l ascorbic acid, 1 mg/l BAP, 0.1 mg/l NAA and 0.02 mg/l GA3 responded the best for shoot induction and proliferation. The highest percentage of shoot induction (77.5 %) and number of shoots per explants (18) was obtained from Orphan cultivar. The HCMS liquid media supplemented with 100 mg/l ascorbic acid, 0.6 mg/l BAP, 0.1 mg/l NAA and 0.1 mg/l GA3 was the optimized media for shoot elongation. The length of shoot obtained after 4 weeks of culture were 43.5 mm, 37.2 mm and 29.8 mm for Orphan, Masui Dauphine and A134, respectively. The MS liquid media without plant growth regulator supplement (MR0) was the best rooting media for all cultivars. The best rooting (85 %) was obtained from Orphan. Rooted plantlets were successfully acclimatized on soil. Study on genetic variation showed that the regeneration system retained genetic stability. Twelve primers produced 893 identical bands ranged between 300 and 1500 bp from 19 plants sample. The study concluded that selected cultivars of this study may be cultivated for fig production in Malaysia and in vitro regeneration might have high potential to be applied in the future.,Certification of Master's/Doctoral Thesis" is not available | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Bangi | - |
| dc.relation | Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina | - |
| dc.rights | UKM | - |
| dc.subject | Germplasm resources | - |
| dc.subject | Plant -- Malaysia | - |
| dc.title | Germplasm screening, in vitro regeneration and molecular studies of selected fig (Ficus carica L.) cultivars for Malaysian climate | - |
| dc.type | Theses | - |
| dc.format.pages | 163 | - |
| dc.identifier.callno | QK981.M826 2018 3 tesis | - |
| dc.identifier.barcode | 003424(2018) | - |
| Appears in Collections: | Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina | |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_100868+SOURCE1+SOURCE1.0.PDF Restricted Access | 1.67 MB | Adobe PDF |  View/Open |
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