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Title:	Two-stage biohydrogen and methane production from palm oil effluent (POME) using anaerobic sludge
Authors:	Mariatul Fadzillah Mansor (P49141)
Supervisor:	Jamaliah Md Jahim, Prof. Dr.
Keywords:	Anaerobic bacteria
Issue Date:	11-Jul-2017
Description:	Anaerobic digestion from palm oil mill effluent (POME) has been used for a waste water treatment and gained an energy production of biohydrogen and methane. This study demonstrated two-stage production of biohydrogen and methane using anaerobic sludge as an inoculum and POME as a substrate. In the initial stage, study was focused on pre-treatment of anaerobic sludge and POME to develop an acetogenesis system. The acid pre-treatment (pH 3.0) was showing a significance effect on anaerobic sludge while heat pre-treatment (80°C) and untreated POME showed most similar results in biohydrogen production. Batch study for biohydrogen production was then performed and analysis by Gompertz equation indicated that using untreated POME gave higher production rate than using heated POME. Therefore, untreated POME was chosen to be a substrate in further experiments. The acidic treated anaerobic sludge was acclimatized for few months using series of diluted POME to be used as an inoculum. Continuous fermentation of biohydrogen was conducted in 2 liter continuous stirred tank (CSTR). Initial set-up for CSTR consist of 50% acclimatized anaerobic sludge and 50% diluted POME had HRT 3 days operated at 35°C with initial pH set at 7. The system failed to produce biohydogen in the initial runs where methane gas were evolved as the process continued. Few strategies were applied such as changing the inoculum treatment and POME source, operated at low HRT and varying temperature. Increasing the temperature to thermophilic (55°C) had improved production of biohydrogen to achieve production rate 1.16 l/l.d at steady state. As the system free from methane, operating parameters such as pH (pH range from 6.1-6.2 and 5.7-5.8), POME concentration (50%, 75% and 100%) and HRT (2, 3 and 4 days) were manipulated to enhanced biohydrogen production. The highest biohydrogen was produced when CSTR was operated at 55°C, pH range after feeding 5.7-5.8, 75% diluted POME and at HRT 2 days. Biohydrogen production rate was 1.72 l/l.d at OLR 37.5 g COD/l.d with acetic and butyric acid were main soluble metabolites. In the second stage fermentation of methane, effluent from biohydrogen reactor was collected to be a substrate. This study was conducted in 5 liter CSTR operated continuously in series with biohydrogen reactor at temperature 35°C. The performance of methane production was observed based on different characteristics in biohydrogen effluent at HRT 10.5 days. The only parameter studied in production of methane was HRT 10.5, 7 and 3.5 days. In this study, the highest production rate of methane of 0.79 l/ l. d was achieved when CSTR was operated at HRT 7 days and OLR 9.69 g COD/ l. d. Almost 80% soluble metabolites were utilized and 70% COD was removed. Therefore, in this study, fermentation of anaerobic sludge and POME was successfully gained two valuable products biohydrogen and methane continuously in the CSTR in series with maximum utilization of POME.,Ph.D.
Pages:	119
Call Number:	TD475.M347 2017 3 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

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