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https://ptsldigital.ukm.my/jspui/handle/123456789/460450| Title: | Effects of ethyl acetate extract of zingiber zerumbet rhizomes on hydogen-peroxide induced abnormality of rat red blood cells |
| Authors: | Mohd Fuad Rahmat Sam (P54397) |
| Supervisor: | Siti Balkis Binti Budin, Professor Dr. |
| Keywords: | Zingiber zerumbet Antirheumatic Anti-inflammatory Antispasmodic Antitumour Dissertations, Academic -- Malaysia |
| Issue Date: | 15-Aug-2014 |
| Description: | Scientific studies have shown that Zingiber zerumbet possesses antirheumatic, anti-inflammatory, antispasmodic and antitumour effects. The aim of the present study was to evaluate the protective effects of ethyl acetate extract of Z. zerumbet rhizome against in vitro oxidative damage in rats red blood cells (RBCs) induced by 2 mM of hydrogen peroxide (H2O2). The phytochemical analysis of the extract, degree of haemolysis, oxidative stress status and morphological changes of RBCs were examined. The extract compounds were determined using Gas Chromatography–Mass Spectrometry (GC-MS) and Zerumbone was identified as the highest constituency (51.57%). The 50, 25, 12.5 and 6.25 μg/ml concentrations of ethyl acetate extract of Z. zerumbet rhizome did not exhibit significant differences in the percentage of haemolysis and malondialdehyde (MDA) level compared to the control group. Therefore, all four doses were considered as the optimum doses and were used for further analysis. The total phenolic content and antioxidant activities using Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay in these four optimum doses were significantly lower (p<0.05) compared to the standard antioxidant, Butylated Hydroxytoluene (BHT). In the in vitro study, rat RBCs were incubated with all optimum doses for 1 hour, followed by 2 mM H2O2 for 2 hours and were known as 6.25 μg/ml + H2O2, 12.5 μg/ml + H2O2, 25 μg/ml + H2O2 and 50 μg/ml + H2O2 group. While, RBCs that were incubated in NaCl, H2O2, and BHT + H2O2 acted as normal, negative and positive control groups, respectively. The haemolysis percentage, MDA and protein carbonyl (PC) levels were significantly lower (p<0.05) in the 6.25 μg/ml + H2O2 group compared to the H2O2 group. Contrarily, the haemolysis percentage, MDA and PC levels were significantly higher (p>0.05) in the 50 μg/ml + H2O2 group compared to the H2O2 group. Meanwhile, reduced glutathione (GSH) level, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in all the extract groups did not differ significantly compared to the H2O2 group. Morphological changes of RBCs to echinocyte form due to H2O2 exposure were prevented effectively in the 6.25 μg/ml + H2O2 group only. This study revealed that 6.25 μg/ml ethyl acetate extract Z. zerumbet rhizome could protect the oxidative damage of rat RBCs induced by H2O2.,Ijazah Sarjana Sains (Biomedikal) |
| Pages: | 148 |
| Call Number: | QY402.M6978e 2014 9 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Health Sciences / Fakulti Sains Kesihatan |
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|---|---|---|---|---|
| ukmvital_97253+Source01+Source010.PDF Restricted Access | 171.4 kB | Adobe PDF |  View/Open |
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