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https://ptsldigital.ukm.my/jspui/handle/123456789/457970Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Prof. Dr. Siti Aishah Md. Ali | - |
| dc.contributor.author | Nurhayati Munawer. (P53948) | - |
| dc.date.accessioned | 2023-09-13T01:48:55Z | - |
| dc.date.available | 2023-09-13T01:48:55Z | - |
| dc.date.issued | 2014-04-10 | - |
| dc.identifier.other | ukmvital:81282 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/457970 | - |
| dc.description | Penanda molekul mungkin berguna dalam diagnosis tumor disamping menentukan prognosis dan meramal respons pesakit terhadap terapi. Kajian ini bertujuan untuk menentukan pengekspresan penanda molekul ERα, ERβ, MIB-1, TOP2A, SMA dan SMMHC pada sel epitelium, mioepitelium dan stroma pada tumor payudara benigna dan malignan. Hubungan ekspresinya dengan pembolehubah patologi klinikal turut dikaji. Sebanyak 22 hiperplasia duktal, 66 karsinoma duktal invasif (invasive ductal carcinoma, IDC) dan 191 tumor fibroepitelium dikaji dengan menggunakan teknik imunohistokimia bagi penanda molekul ini. Status amplifikasi gen HER-2 dan TOP2A ditentukan ke atas sampel IDC menggunakan teknik penghibridan in situ kromogenik (CISH). Bilangan kes yang positif ERα dan ERβ di sel epitelium didapati lebih banyak hadir pada tumor benigna berbanding malignan (p=0.017, p=0.014). Pengekspresan MIB-1 di sel epitelium IDC didapati mempunyai hubungan dengan peningkatan gred tumor (p=0.026), positiviti ER (p=0.042) dan positiviti PR (p=0.004). Sebanyak 18.5% (12/65) dan 14% (9/64) kes IDC masing-masing menunjukkan amplifikasi gen HER-2 dan TOP2A. Amplifikasi gen HER-2 menunjukkan hubungan signifikan dengan gred histologi tinggi dan c-erbB-2 positif (p<0.05). Tiga kes yang menunjukkan amplifikasi bagi kedua-dua HER-2 dan TOP2A didapati hadir pada tumor yang mempunyai saiz tumor besar (>2 cm) dan nodus limfa positif, tetapi tidak signifikan secara statistik. Bagi tumor fibroepitelium, pengekspresan penanda molekul di epitelium menunjukkan corak yang sama dengan tumor epitelium. Pengekspresan kedua-dua klon ER menurun dengan gred manakala positiviti MIB-1 dan TOP2A meningkat dengan gred histologi. Namun pengekspresannya tidak menunjukkan hubungan signifikan dengan data klinikal. Hubungan signifikan hanya ditemui pada komponen stroma. Pengekspresan ERβ di stroma meningkat dengan gred tumor dari fibroadenoma hingga tumor filodes malignan (p=0.000). Sel stroma tumor filodes yang positif ERβ didapati mempunyai hubungan signifikan dengan pengekspresan SMA (p=0.047) menunjukkan peningkatan pembezaan sel stroma, tetapi tidak signifikan dengan gred tumor. Peningkatan ekspresi MIB-1 di stroma dengan gred histologi fibroadenoma dan tumor filodes selari dengan peningkatan indeks proliferasi sel stroma dari benigna kepada malignan. Ia juga selari dengan peningkatan min saiz tumor. Hasil kajian ini boleh menyokong kenyataan bahawa kedua-dua epitelium dan stroma tumor fibroepitelium adalah neoplastik tetapi tumor ini mungkin mempunyai tapak jalan neoplastik yang berbeza dengan tumor epitelium seperti IDC. Walaubagaimanapun, patogenesis bagi tumor filodes adalah kurang jelas. Kajian ini juga berpendapat teknik imunohistokimia sahaja tidak cukup untuk meramal hasil klinikal bagi tumor jenis ini. Kajian genetik adalah dicadangkan untuk membandingkan tumor fibroepitelium dengan neoplasma stroma yang lain seperti sarkoma untuk memahami patogenesis kumpulan tumor ini.,Molecular markers may be useful in tumour diagnosis as well as determining prognosis and predicting response of patients to therapy. The aims of this study were to evaluate the expression of molecular markers, ERα, ERβ, MIB-1, TOP2A, SMA and SMMHC in epithelial, myoepithelial and stromal cells of benign and malignant breast tumours. The expressions of these markers were then correlated with clinicopathological variables. A total of 22 ductal hyperplasias, 66 invasive ductal carcinomas (IDC) and 191 fibroepithelial tumours were immunohistochemically stained for these markers. The status for amplification of HER-2 and TOP2A genes on IDC samples were determined using chromogenic in situ hybridisation (CISH). The number of positive cases of ERα and ERβ in the epithelial cells were seen more in benign compared to malignant tumours (p=0.017, p=0.014). The expression of MIB-1 in epithelial cells of IDC was found to be associated with increased tumour grade (p=0.026), ER positivity (p=0.042) and PR positivity (p=0.004). A total of 18.5% (12/65) and 14% (9/64) cases of IDC showed HER-2 and TOP2A gene amplification, respectively. The amplification of HER-2 was significant with high histological grade and c-erbB-2 positive (p<0.05). Three cases that showed both HER-2 and TOP2A amplification were present in tumour with large tumour size (>2 cm) and positive lymph nodes, but was not found to be statistically significant. For fibroepithelial tumours, expressions of molecular markers in epithelium showed the same pattern as in epithelial tumour. The expressions of both ER were decreased with grade while positivity of MIB-1 and TOP2A were increased with histological grade. However, their expressions were not found to be significant with clinical data. Significant association has been found in stromal component. The expression of ERβ in the stroma was increased with tumour grade from fibroadenoma to malignant phyllodes tumour (p=0.000). Positive ERβ in stromal cells of phyllodes tumour was significantly associated with the expression of SMA (p=0.047) but was not significant with tumour grade. Increased MIB-1 expression in stroma with histological grade of fibroadenoma and phyllodes tumour was in line with increased proliferation index from benign to malignant. It was also consistent with increased mean of tumour size. Both epithelium and stromal component were neoplastic, but only stromal component showed association with clinical data. The findings in this study may support that both of epitelium and stroma of fibroepitelial tumour are neoplastic but this tumour may have a different neoplastic pathway than epithelial tumour such as IDC. However, the pathogenesis of phyllodes tumour is poorly understood. This study confine that immunohistochemistry alone does not significantly predict clinical outcome for this tumour. Genetic study is recommended to compare fibroepithelial tumour with other stromal neoplasm such as sarcoma to understand the pathogenesis of this group of tumour.,Master | - |
| dc.language.iso | may | - |
| dc.publisher | UKM, Kuala Lumpur | - |
| dc.relation | Faculty of Medicine / Fakulti Perubatan | - |
| dc.rights | UKM | - |
| dc.subject | Penanda molekul | - |
| dc.subject | Tumor payudara benigna dan malignan | - |
| dc.subject | Teknik imunohistokimia | - |
| dc.subject | Pathology. | - |
| dc.title | Pengekspresan penanda molekul ke atas tumor payu dara benigna dan malignan menggunakan teknik imunohistokimia dan penghibridan in situ kromogenik | - |
| dc.type | theses | - |
| dc.format.pages | 181 | - |
| dc.identifier.callno | QZ20.5 .N974p 2014 9HUKM | - |
| dc.identifier.barcode | 001670 | - |
| Appears in Collections: | Faculty of Medicine / Fakulti Perubatan | |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_81282+SOURCE1+SOURCE1.0.PDF Restricted Access | 5.14 MB | Adobe PDF |  View/Open |
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