1. UKM Learning and Research Repository
2. Master Thesis / Tesis Master
3. Faculty of Pharmacy / Fakulti Farmasi

Title:	In vitro inhibitory effects of compounds from several medicinal plants on prostaglandin E2 (PGE2) production in human plasma and Platelet-Activating Factor (PAF) receptor binding
Authors:	Siti Norafiqah Mohd Sazali (P78683)
Supervisor:	Juriyati Jalil, Prof. Dr.
Keywords:	Plants Medicinal Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	26-Mar-2019
Description:	Prostaglandin E2 (PGE2) and platelet-activating factor (PAF) are among mediators that involved in inflammation. Inflammation is a biological process characterised by redness, swelling, and pain. Thousands of plants in Malaysia have been used for medicinal purposes including Artocarpus, Zingiber, Phyllanthus and Labisia species. Folks used these medicinal plants to treat inflammation traditionally. However, some of these medicinal plants is not well studied. Futhermore, commercialized antiinflammatory drugs were reported to give side effect. Therefore, new anti-inflammatory agent can be developed as an alternatives to the conventional drugs. In this study, seven flavonoids from Artocarpus species namely, artocarpin (28), isobavachalcone (92), cycloheterophyllin (93), 2',4'-dihydroxy-4-methoxy-3'-prenyldihydrochalcone (94), 4',5-dihydroxy-6,7-(2,2-dimethylpyrano)-2'-methoxy-8--8-γ,γ-dimethylallylflavone (95), artonin E (96) and oxyresveratrol (97), eight compounds from Zingiber species, 6- gingerol (44), 8-gingerol (98), 10-gingerol (99), 6-shogaol (45), 8-shogaol (100), 10- shogaol (101), curcumin (102) and (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (103), four compounds from Phyllanthus species, phyllantin (106), hypophyllantin (107), niranthin (108) and corilagin (77) and four compounds from Labisia species, demethylbelamcandaquinone B (109), iriresorcinol (110), 5-(Z-nonadec-14-enyl) resorcinol (111) and belamcandol B (112) were investigated for their inhibitory effects on the production of PGE2 in human whole blood induced by lipopolysaccharide (LPS) and PAF receptor binding with rabbit platelets using 3H-PAF as a ligand. Indomethacin and cedrol were used as positive control, respectively. The results showed that 18 compounds gave more than 50% inhibitory effects on the production of PGE2 at concentration 10 ?g/mL. Among the active compound, 11 compounds including compound 28, 94, 95, 96, 99, 102, 106, 107, 108, 109 and 111 showed strong concentration-dependent inhibitory activity with IC50 values of 11.66, 9.31, 7.04, 8.98, 10.27, 13.74, 15.08, 10.02, 5.37, 5.13 and 9.46 ?M, respectively. IC50 values of these compound were comparable to that of indomethacin (IC50=15.08 ?M). Meanwhile, for the PAF receptor binding assay, 13 compounds showed more than 50% inhibitory activity at concentration of 18.2 ?g/mL. Compound 93, 77 and 109 showed strong concentration-dependent inhibitory activity with IC50 values of 6.83, 10.89 and 8.67 ?M, respectively and these values were comparable with cedrol (IC50=11.44 ?M). From this study, it can be concluded that these compounds are promising natural product candidates that can be developed as anti-inflammatory drugs.,Master of Science
Pages:	114
Call Number:	9 Tesis QV766. S623i 2019
Publisher:	UKM, Kuala Lumpur
Appears in Collections:	Faculty of Pharmacy / Fakulti Farmasi

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.