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Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578652
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dc.contributor.authorKhwanduean Rattana
dc.contributor.authorSupavee Sangchanjiradet
dc.date.accessioned2023-11-06T03:05:10Z-
dc.date.available2023-11-06T03:05:10Z-
dc.date.issued2017-11
dc.identifier.issn1511-3701
dc.identifier.otherukmvital:116102
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578652-
dc.descriptionA study was conducted to elucidate the effects of using different media (MS, ½MS, VW) supplemented with different combinations of 15% coconut water, 10% potato extract and 5% mashed banana with an extra 0.2% activated charcoal on seed germination of Dendrobium signatum Rchb.f. in in vitro cultures. The results showed that the most suitable media, providing 100% seed germination, were MS added to 10% potato extract, ½MS supplemented with 10% potato extract and MS added to 5% mashed banana. Young plantlets were then transferred to MS and ½MS medium supplemented with various kinds of cytokinin including BA, Kinetin and TDZ at the concentrations of 0, 1 and 2 mg/l, and combined with 0 and 0.5 mg/l of NAA for plantlet development. The results showed that young shoots could be differentiated and regenerated into plantlets in all the treatments. The results revealed that the most suitable medium for shoot proliferation and root induction was ½MS medium with 2 mg/l BA added and combined with 0.5 mg/l NAA.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=1&journal=JTAS-40-4-11
dc.rightsUKM
dc.subjectDendrobium
dc.subjectIn vitro culture
dc.subjectCytokinin
dc.subjectSeed germination
dc.subjectOrganic supplements
dc.titleMicropropagation of dendrobium signatum rchb.f.
dc.typeJournal Article
dc.format.volume40
dc.format.pages577-586
dc.format.issue4
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