1. UKM Learning and Research Repository
  2. Journal Article / Artikel Jurnal
  3. Journal Content Pages/ Kandungan Halaman Jurnal
Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578470
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMuid S (UITM)
dc.contributor.authorFroemming G. R. A (UITM)
dc.contributor.authorAli A. M (UNISZA)
dc.contributor.authorNawawi H (UITM)
dc.date.accessioned2023-11-06T03:02:18Z-
dc.date.available2023-11-06T03:02:18Z-
dc.date.issued2017-07
dc.identifier.issn0128-7680
dc.identifier.otherukmvital:115952
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578470-
dc.descriptionHuman umbilical vein endothelial cells (HUVECs) were cultured on microcarrier beads to accommodate different experiment apparatus such as rotating wall vessel. In this study, fluid operating apparatus (FPA) was used. However, the effect of inflammation and endothelial activation biomarkers in HUVECs cultured on different culture surface and containers are not well established. The effects of temperature changes on these biomarkers in HUVECs grown in FPA, a spaceflight hardware, are still unclear. The objective of this study was to compare the protein and gene expression of inflammation and endothelial activation biomarkers in (i) HUVECs cultured on microcarrier beads in conventional culture flask (CCFMC) vs. conventional culture flask (CCF) (ii) HUVECs cultured on microcarrier in FPA (FPAMC) vs. CCFMC and (iii) HUVEC cultured in FPAMC with ideal temperature (37°C) (FPAMC) vs. simulated space travel temperature(25-37°C), (FPAMC-ST). sICAM-1 and sVCAM-1protein expression in HUVECs grown in CCFMC were higher than CCF. FPAMC had higher IL-6, TNF-?, ICAM-1, VCAM-1, e-selectin, NF?B and eNOS gene expression than in CCFMC. FPAMC-ST had higher ICAM-1 and e-selectin protein expression than FPAMC- in ideal temperature. HUVECs are cultured onto microcarrier in simulated space flight temperature compared with ideal temperature had higher protein expression of sICAM-1 and e-selectin but the protein and gene expression of other biomarkers of inflammation and endothelial activation are comparable. This suggests that differences in culture surface and container are have an impact on the expression of inflammation and adhesion molecule by HUVECs.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=2&journal=JST-25-S-7
dc.rightsUKM
dc.subjectAdhesion molecules
dc.subjectEndothelial cells
dc.subjectFluid processing apparatus (FPA)
dc.subjectInflammation
dc.subjectMicrocarrier beads
dc.titleImpact of container, temperature and microcarriers on inflammation and endothelial activation in human endothelial cells
dc.typeJournal Article
dc.format.volume25
dc.format.pages263-274
dc.format.issueSpecial Issue
Appears in Collections:Journal Content Pages/ Kandungan Halaman Jurnal

Files in This Item:
File Description SizeFormat 
ukmvital_115952+Source01+Source010.PDF579.91 kBAdobe PDFThumbnail
View/Open
Show simple item record Recommend this item


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.