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https://ptsldigital.ukm.my/jspui/handle/123456789/578366| Title: | Effect of mevalonic acid (MVA) and linalool as a precursor in enhancement of limonene in citrus grandis osbeck albedo tissue culture |
| Authors: | Nik Norulaini N. A. R (USM) Thamare K. M (USM) Zarina Z (UNIMAP) Tengku Norsalwani T. L (USM) |
| Keywords: | Mevalonic acid (MVA) Linalool Precursor Limonene production Citrus grandis Osbeck Albedo tissue culture |
| Issue Date: | Feb-2018 |
| Description: | The effects of melavonic acid (MVA) and linalool as precursors in the production of limonene and linalool on Citrus grandis Osbeck callus tissues were investigated. MVA and linalool were used as precursors to stimulate limonene production in the biosynthetic pathway. This study proved that low concentrations of MVA (0.077 mM to 1.557 mM) and linalool (0.056 mM to 1.117 mM) were able to produce limonene when tested on callus tissues for 7 to 35 days. The aim was to determine the highest accumulation of both limonene and linalool. The highest production of limonene obtained was 0.97 ppm on day 28 when the tissues were treated with linalool and 1.50 ppm on day 35 with the addition of 0.077 mM MVA. On the other hand, linalool concentration reached a maximum of 2.88 ppm on day 7 with tissues treated with 0.077 mM MVA. As the culture period lengthened, the limonene level increased from 0.76 ppm at day 7 to 1.82 ppm on day 28, whereas linalool concentration decreased steadily from 2.88 ppm at day 7 to 1.55 ppm at day 35. This is due to the bioconversion of linalool to limonene. The best result for precursortreated tissues was at 0.838 mM linalool, where the limonene level achieved was 0.97 ppm on day 28. The production of limonene and linalool using low precursor concentrations within a short period of time is favourable as it has good market value. |
| News Source: | Pertanika Journals |
| ISSN: | 1511-3701 |
| Volume: | 41 |
| Pages: | 101-114 |
| Publisher: | Universiti Putra Malaysia Press |
| Appears in Collections: | Journal Content Pages/ Kandungan Halaman Jurnal |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_115871+Source01+Source010.PDF | 375.53 kB | Adobe PDF |  View/Open |
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