1. UKM Learning and Research Repository
  2. Journal Article / Artikel Jurnal
  3. Journal Content Pages/ Kandungan Halaman Jurnal
Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578240
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSuvapa Yottakot
dc.contributor.authorVichai Leelavatcharamas
dc.date.accessioned2023-11-06T02:59:29Z-
dc.date.available2023-11-06T02:59:29Z-
dc.date.issued2019-08
dc.identifier.issn1511-3701
dc.identifier.otherukmvital:115765
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578240-
dc.descriptionNowadays polylactic acid (PLA) is used extensively with respect to environmental concern and good practice in solid-waste management, as food packaging. However, the study on biodegradation of PLA-packaging is very limited. In this work, isolation, identification and optimisation of biomass production of PLA-packaging-degrading actinomycete were carried out. The isolate KKU215 gave the highest cell density in basal medium containing 1.0 g/L PLA-packaging as sole carbon source after 4-week incubation. The weight loss of PLA-packaging degraded by the isolate was 2.65%. The results from scanning electron microscopy analysis indicated that the isolate KKU215 could clearly degrade PLApackaging. The degradation of pure PLA by KKU215 as clear zone formation on emulsified PLA agar plate was observed. The 16S rDNA gene sequence of the isolate KKU215 was related to the genus Streptomyces. Thus, the isolate KKU215 was assigned as Streptomyces sp. KKU215. Concentration of yeast extract, initial pH value, temperature and agitation speed for PLA-packaging degradation were optimised by response surface methodology with Box-Behnken design. The highest growth of Streptomyces sp. KKU215 was found in basal medium with 3.26% yeast extract, initial pH value of 7.69, and agitated at 149 rpm at 33.7°C. Streptomyces sp. KKU215 when cultured in the optimal conditions could enhance PLA-packaging weight loss from 2.65% to 84.04%, which was 81.39% higher than the unoptimised conditions. Up to date, there is no report of Streptomyces species, similar with Streptomyces sp. KKU215 capable of degrading PLA. Therefore, Streptomyces sp. KKU215, a novel polylactic acid-packaging-degrading actinomycete is a promising strain for use in biodegradation of PLA-packaging.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journals
dc.relation.urihttp://www.pertanika.upm.edu.my/regular_issues.php?jtype=1
dc.rightsUKM
dc.subjectBiodegradation
dc.subjectBioplastic
dc.subjectBox-Behnken design
dc.subjectOptimisation
dc.subjectPolylactic acid (PLA)-packaging
dc.subjectResponse surface methodology (RSM)
dc.subjectStreptomyces sp. KKU215
dc.titleIsolation and Optimisation of Polylactic Acid (PLA)-packagingdegrading Actinomycete for PLA-packaging Degradation
dc.typeJournal Article
dc.format.volume42
dc.format.pages1111-1129
dc.format.issue3
Appears in Collections:Journal Content Pages/ Kandungan Halaman Jurnal

Files in This Item:
File Description SizeFormat 
ukmvital_115765+Source01+Source010.PDF2.38 MBAdobe PDFThumbnail
View/Open
Show simple item record Recommend this item


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.