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Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/578052
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dc.contributor.authorShazrul Fazry (Universiti Kebangsaan Malaysia)
dc.contributor.authorMalina Kumaran (Universiti Kebangsaan Malaysia)
dc.contributor.authorNahid Khalili (Universiti Kebangsaan Malaysia)
dc.contributor.authorIbrahim Mahmood (Universiti Kebangsaan Malaysia)
dc.contributor.authorHerryawan Ryadi Eziwar Dyari (Universiti Kebangsaan Malaysia)
dc.contributor.authorNur Hidayah Jamar (Universiti Kebangsaan Malaysia)
dc.contributor.authorNor Azian Abdul Murad (Universiti Kebangsaan Malaysia)
dc.date.accessioned2023-11-06T02:58:09Z-
dc.date.available2023-11-06T02:58:09Z-
dc.date.issued2019-10
dc.identifier.issn0128-7680
dc.identifier.otherukmvital:129816
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/578052-
dc.descriptionThe zebrafish (Danio rerio) has become a prevailing vertebrate model for developmental biology studies due to its ease of care, rapid embryogenesis stages development and translucent embryos. In this studies, ATM Kinase and MRN complex role as DNA damage response proteins during embryogenesis was examined by using specific MRN complex (Mirin) and ATM Kinase inhibitors (Ku60019 and Ku55933). To create DNA lesions in zebrafish, embryos at mid-blastula transition (MBT) stage were exposed to inhibitors (Mirin, Ku60019 or Ku55933) and later exposed to UVC irradiation wavelength of between 100 to 280 nm. Hatching but with visible physical deformation was observed for embryos treated with Mirin, Ku60019 or Ku55933 and UVC exposure at concentration of 3µM, 1.5 nM and 3nM or lower, respectively up to 72 hours-post fertilisation (hpf). On the other hand, no deformities were observed for all control as well as mock treated embryos. This study confirmed that DNA damage response proteins are crucial during embryo development to prevent undesired abnormal biological development. Thus, it is proven that protein inhibitors are a cheaper alternative in valuating specific protein roles during embryogenesis compared to both genomic and transcription modification tools.
dc.language.isoen
dc.publisherUniversiti Putra Malaysia Press
dc.relation.haspartPertanika Journal of Social Sciences & Humanities
dc.relation.urihttp://www.pertanika.upm.edu.my/pjst/browse/archives?journal=JST-27-4-10
dc.rights(c) Universiti Putra Malaysia Press
dc.subjectATM Kinase
dc.subjectDanio rerio
dc.subjectDNA damage
dc.subjectMRN complex
dc.subjectZebrafish
dc.titleMRN complex and ATM Kinase inhibitors impacts towards UVC-Treated Zebrafish Embryonic Development
dc.typeJournal Article
dc.format.volume27
dc.format.pages1615-1624
dc.format.issue4
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