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https://ptsldigital.ukm.my/jspui/handle/123456789/519886| Title: | Hepatoprotective activity of polygonum minus essential oil against cisplatin-induced liver injury in sprague dawley rats |
| Supervisor: | Siti Balkis Budin, Prof. Dr. |
| Keywords: | Liver Hepatoprotective Oils Volatile |
| Issue Date: | 27-Jul-2020 |
| Description: | Cisplatin is a chemotherapeutic agent widely used in treating various types of cancer. However, its usage can cause various adverse effects including hepatotoxicity which involved inflammation, apoptosis and DNA damage. This study aimed to determine the protective effects of Polygonum minus essential oil (PmEO) towards cisplatininduced hepatotoxicity in rats. Extraction of P. minus essential oil was performed using hydrodistillation technique and its compound was identified using gas chromatography-mass spectrometry (GC-MS). A total of 37 compounds were identified; with aldehyde (52.47%), sesquiterpene (17.9), organic acid (7.24%), aliphatic compound (3.04) and alcohol (2.38%). A total of forty-two male rats were randomly divided into seven groups: control, cisplatin, β-caryophyllene 150 mg/kg (BCP), PmEO 100 mg/kg + cisplatin (PmEO100CP), PmEO 200 mg/kg + cisplatin (PmEO200CP), PmEO 400 mg/kg + cisplatin (PmEO400CP) and PmEO 400 mg/kg (PmEO400). Rats in the BCP, PmEO100CP, PmEO200CP, PmEO400CP and PmEO400 group received respected treatment orally for the duration of 14 consecutive days. At day 15, all groups except the control group and PmEO400 were introduced with a single dose of cisplatin (10 mg/kg) intraperitoneally. Three days later, rats were sacrificed, blood and liver were obtained for determination of enzymes, morphological changes, oxidative stress status, inflammatory cytokines, cytochrome C protein expression and apoptotic marker gene expression. Results showed that PmEO100CP pretreatment protects against cisplatin-induced liver damage by significantly reduced (p<0.05) transaminase enzymes compared to cisplatin group along with liver oxidative stress markers including malondialdehyde (MDA), 8-OHdG and protein carbonyl and in turn significantly increased the antioxidant status of glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase compared to cisplatin group (p<0.05). In addition, PmEO100CP also significantly lowered the inflammatory markers; TNF-α, IL-1α, -1β and -6 while IL-10 were markedly increased compared to cisplatin group (p<0.05). Pretreatment of essential oil on PmEO100CP group downregulated the expression of apoptotic markers p53, caspase 8, Bax, cytochrome C, APAF-1, caspase 9 and caspase 3 while antiapoptotic marker Bcl2 gene expression was markedly upregulated. The examination of light and electron microscope demonstrated a well preserved and organized hepatocytes also a minimum morphological changes in PmEO100CP group. The results also demonstrated that PmEO100CP has significantly better protective effects on cisplatin-induced hepatic toxicity than PmEO200CP and PmEO400CP. (p<0.05) and did not show any significant difference compared to the BCP group. In conclusion, our results suggested that P. minus essential oil at a dose of 100 mg/kg may protect cisplatin-induced liver toxicity via inhibition of oxidative stress, inflammation and apoptosis.,Doctor Of Philosophy |
| Pages: | 271 |
| Call Number: | 9 Tesis QV786.5.O4N893h 2020 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Health Sciences / Fakulti Sains Kesihatan |
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|---|---|---|---|---|
| ukmvital_118179+Source01+Source010.PDF Restricted Access | 3.09 MB | Adobe PDF |  View/Open |
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