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https://ptsldigital.ukm.my/jspui/handle/123456789/519872Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Noraziah Mohamad Zin, Associate Professor Dr. | - |
| dc.contributor.author | Muhanna Mohammed Ahmad Alshaibani (P63276) | - |
| dc.date.accessioned | 2023-10-17T09:30:40Z | - |
| dc.date.available | 2023-10-17T09:30:40Z | - |
| dc.date.issued | 2017-09-05 | - |
| dc.identifier.other | ukmvital:99880 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/519872 | - |
| dc.description | Methicillin resistant Staphylococcus aureus (MRSA) expresses a widespread of virulence factors and antimicrobial resistance determinants that lead to increased mortality and morbidity, and health care costs. The endophytic Streptomyces that resides in the living tissues of the plants are alternative medicine that can be used as anti-MRSA agents. This study aimed to determine the anti-MRSA activity of the bioactive compounds derived from endophytic Streptomyces sp. SUK 25 which was isolated from the root tissues of Zingiber spectabile. The production of secondary metabolites was optimized through Thornton's broth medium. Isolation, purification, and identification of the bioactive compounds were carried out using TLC, column chromatography, analytical and preparative HPLC, and Fourier Transform Infrared (FT-IR). The chemical structure elucidation analysis was carried out by HR-LC-ESIMS and NMR. During primary and secondary screening, SUK 25 exhibited good activity against several MRSA ATCC strains. Thornton's broth medium was the best culture fermentation medium with low MIC 4 μg/mL and the diameter of zone of inhibition was 16.8 mm. Bioactivity evaluation test against MRSA ATCC 43300 was carried out by using disk diffusion method for thirteen fractions were obtained by column chromatography and TLC methods. The best biological activities were shown from fractions number; F1, F2, F7, F9 and F10 with MICs value ringing from 4 to 16 μg/mL. In this study only fraction 2 (F2) and fraction 7 (F7) were selected for the further isolation, purification and identification. From F2 five compounds were isolated and identified as N-(7-hydroxy-6-methyl-octyl)-acetamide and four diketopiperazine derivatives were they elucidated as four diketopiperazines, identify as cyclo-(L-Valyl- L-Prolyl), cyclo-(Leucyl-Prolyl), cyclo-(Phenylalanine-Prolyl), cyclo-(L-Valyl- Phenylalanine). As well as, from F7 two compounds were isolated and identified as chloramphenicol and cyclo-(L-Tryptophanyl-L-Prolyl). Determination of the gene transcriptional profile by using DNA microarray methods of MRSA ATCC 43300 treated with subinbibitory concentration of compounds chloramphenicol and cyclo-(LValyl- L-Prolyl), revealed significant modulation of gene expression with up regulation of 90 genes and down regulation of 140 genes. Among the up-regulated genes, 23 genes were involved in translation with Chloramphenicol and 19 genes were down regulated involved in translation with cyclo-(L-Valyl-L-Prolyl). In vitro cytotoxicity assay against HepaRG cell line revealed that selected compounds cyclo-(L-Valyl-L-Prolyl) and cyclo-(Phenylalanine-Prolyl), isolated from F2 had no cytotoxic activity with IC50 values of 110 and 103 μg/mL, respectively. On the contrary, compound chloramphenicol isolated from F7 exhibited the highest cytotoxic activity with IC50 value of 22.2 μg/mL. In conclusion; this study demonstrates that endophytic Streptomyces SUK 25 has the ability to produce several natural compounds derivatives with potential bioactivity against MRSA with low MICs value as well as lower cytotoxicity to HepaRG cells. This finding represents a good potential natural source of new drugs antimicrobial compounds for the treatment of some MRSA.,Doktor Falsafah | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Kuala Lumpur | - |
| dc.relation | Faculty of Health Sciences / Fakulti Sains Kesihatan | - |
| dc.rights | UKM | - |
| dc.subject | Methicillin | - |
| dc.subject | Staphylococcus aureus | - |
| dc.subject | Dissertations, Academic -- Malaysia | - |
| dc.title | Isolation, purification and characterization of antimicrobial compounds from endophytic Streptomyces species SUK 25 againts methicillin resistant Staphylococcus aureus | - |
| dc.type | Theses | - |
| dc.format.pages | 287 | - |
| dc.identifier.callno | QY95.A461i 2018 9 | - |
| Appears in Collections: | Faculty of Health Sciences / Fakulti Sains Kesihatan | |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_99880+Source01+Source010.PDF Restricted Access | 3.9 MB | Adobe PDF |  View/Open |
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