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https://ptsldigital.ukm.my/jspui/handle/123456789/519655| Title: | Enhanced crude oil hydrocarbon degradation by bacterial consortium cultureimmobilized onto oil palm empty fruit bunch and sawdust |
| Authors: | Mohammad Daher Khalaf Hazaimeh (P49180 ) |
| Supervisor: | SalmijahSurif, Prof. Dr. |
| Keywords: | Crude oil Consortium culture Palm oil Universiti Kebangsaan Malaysia -- Dissertations |
| Issue Date: | 20-Mar-2014 |
| Description: | Biodegradation of crude oil hydrocarbon by free and immobilized bacterial consortium culture (CC) were studied using sawdust (SD) and oil palm empty fruit bunch (OPEFB). The bacterial CC was a mixture of 9 species microorganism which included six species of Gram-negative bacteria of Stenotrophomonas maltophilia, Alcaligenes faecalis ss faecalis, Pseudomonas fluorescens, Serratia macescens ss marcescens, Flavobacterium johnsoniae and Brevundimonas diminuta and three Gram-positive bacteria of Clavibacter agropyri, Gemella palaticanis and Brevibacterium otitidis. All bacteria were previously isolated from heavy metal contaminated industrial waste water. Preliminary study for crude oil degradation was conducted on 1%, 5% and 10% (v/v) concentration for two weeks incubation. One percent (1%, v/v) concentration showed high degradation activity and survival in crude oil. Thus, this concentration was chosen for crude oil degradation on carrier material of SD and OPEFB. Study on crude degradation by free suspension cells of bacterial CC, 1% (v/v) concentration of crude oil was shown high on its degradation activity with value 95.00±0.98% after 8 week of incubation. The survival of bacterial CC was evaluated using Biolog system and all bacterial CC was able to survive at 8 week of incubation except G. palaticanis, A. faecalis ss faecalis and F.johnsoniae. The cell surface hydrophobicity (CSH) of bacterial CC exhibited the value of 60.30±2.20%. Since CSH is reverse attachment force,study on the biofilm production was conducted and biofilm formation was observed after 24 h of incubation. The highest exopolysaccharide (EPS) production was observed at day 3 incubation at value 61.3±2.10% using Alcian blue dye assay. This suggested the initial attachment of the bacterial CC onto substrates relied on CSH where at day 3 incubation significant production of EPS was detected. The performance of bacterial CC was then tested by immobilizing the bacterial CC onto SD and OPEFBmaterials. Results showed the crude oil degradation activity was increased in immobilized bacterial CC on both materials rather than free cells of bacterial CC after 6 weeks of incubation. The SD and OPEFB gave,increased in value of 15.85% and 17.52%, respectively.When the immobilization of bacterial CC was tested on SD and OPEFB in seawater the degradation of crude oil was significantly increased at 23.43% and 30.57%, respectively compared to free cells of bacterial CC. While, tested in river water (fresh water) the bacterial CC immobilized on SD and OPEFB increased the crude oil degradation at value 22.08% and 27.69%, respectively.The OPEFB showed better degradation activity of crude oil compared to SD in both medium (seawater and river water) by increasing value at 9.32% and 7.19%, respectively. Thus, results in the present study demonstrated the immobilizing of bacterial CC onto cheap carrier materials such SD and OPEFB can be developed as potential method in enhancing the degradation of crude oil hydrocarbon.,Certification of Master's/Doctoral Thesis" is not available |
| Pages: | 198 |
| Call Number: | TD427.P4H349 2014 tesis |
| Publisher: | UKM, Bangi |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi |
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