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Title:	Production of antioxidative bioactive elastin peptide from broiler and spent hen skin
Authors:	Mehdi Nadalian (P54700)
Supervisor:	Abdul Salam Babji, Prof. Dr.
Keywords:	Poultry skin Pharmaceutical industries Universiti Kebangsaan Malaysia -- Dissertations
Issue Date:	9-May-2017
Description:	Poultry by-products are a great economic source that need to be exploited. Poultry skin could be utilized to extract protein particularly elastin, which is often incorporated in the production of functional food, cosmetic industry and regenerative medicine. The elastin extracted from broiler hydrolysates (EBH) and elastin extracted from spent hen hydrolysates (ESHH) were produced using three types of proteases: Elastase, Alcalase and Flavorzym. 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities, and metal chelating activity tests for antioxidant activities of EBH and ESHH were assayed. The hydrolysates were purified by ultrafiltration, gel filtration and reverse phase high performance liquid chromatography (RP-HPLC) and liquid chromatography with matrix- assisted laser desorption/ionization (MALDI-TOF/TOF-MS) and used in identifying their peptide sequences. Results of this study indicated that type of protease affected the degree of hydrolysis (DH), where all of the enzymes showed high rate of hydrolysis at 12h and then gradually decreased. The highest DH (66.45%) among the EBH resulted from 12h and (65.08%) among the ESHH resulted from 12h of incubation with Alcalase and Elastase respectively. The effect of DH on antioxidant activities of EBH and ESHH was determined. With a DH of 47.25%, the EBH-Elastase and a DH 51.89%, the ESHH-Elastase exhibited the maximum of DPPH radical-scavenging activity (55.06%) (p < 0.05). EBH and ESHH treated with Elastase at 4h incubation time show the highest metal chelating activity and ABTS radical scavenging activity (p < 0.05). Among the hydrolysates, EBH-Elastase and ESHH-Elastase, which had the highest antioxidant activities, were further purified using ultrafiltration, gel filtration chromatography and RP-HPLC. EBH-ElastaseIII and papain- ESHH-ElastaseIII with MWCO < 3 kDa had the highest ABTS radical scavenging activity with IC50 o.66 and 0.71 times respectively higher than EBH and ESHH.Gel filtrated fractions with the highest antioxidant activity separated from EBH hydrolysates (EBII) and ESHH hydrolysates (ESHIII) were tested for amino acid composition. The results revealed that both fractions had higher amount hydrophobic and aromatic amino acids than their hydrolysates. The potent fractions obtained from RP-HPLC of Elastin extracted from broiler (EB-II4 fraction) and Elastin extracted from spent hen (ESH-III2 fraction) hydrolysates showed DPPH radical scavenging activity. Peptide sequences of antioxidative peptide from EBH and ESHH were identified using MALDI-TOF/TOF-MS. The peptide sequences for EBH were identified as GAHTGPRKPFKPR, GMPGFDVR and ADASVLPK and for ESHH were identified as AVWTSGMD, GPMGPKGMPGYK, SENPGLHR and GVALMSTNQLVAR. Both peptides had hydrophobic amino acid residue responsible for their antioxidant activity. The results of the study are the first report on the elastin extracted from poultry skin hydrolysis with enzymes could provide the peptide with antioxidant activity. In conclusion EBH and ESHH have the potential as a natural functional ingredient in food and pharmaceutical industries.,Certification of Master's/Doctoral Thesis" is not available
Pages:	135
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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