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https://ptsldigital.ukm.my/jspui/handle/123456789/500017| Title: | Novel zwitterionic betaine/cholesterol vesicle hybridized with nanoparticles |
| Authors: | Radi Jadem Mayouf Alenaizi (P70127) |
| Supervisor: | Shahidan Radiman, Prof. Dr. |
| Keywords: | Surfactant Drug Nanoparticles Peptides |
| Issue Date: | 15-May-2017 |
| Description: | In this study, zwitterionic carboxylate betaine surfactant and cholesterol are used in preparing vesicle system in order to develop surfactant/lipid system for drug delivery applications. These materials are biodegradable, nontoxic, obtained from renewable natural sources, with the existence of the amino acid moiety in the betaine head-group. Moreover, this novel system is used as a template for hybridizing vesicles with hydrophobic metal and metal oxide nanoparticles (i.e. Au, Ag, and Fe3O4), which can introduced functionality in the targeting and controlling applications. Ternary phase diagram was used to determine the behavior and prolonged stability of the betaine surfactant/cholesterol/water system in water rich corner. The hybrid assemble has been synthesized with embedded nanoparticles using hydration of the composed thin film prepared by multi stage reverse phase evaporation. The prepared vesicles and hybrid vesicle samples were characterized by transmission electron microscopy (TEM), high resolution transmission electron microscopy with energy dispersive x-ray (HRTEM-EDX), atomic force microscopy (AFM), dynamic light scattering (DLS), differential scanning calorimetry (DSC), zeta potential, vibrating sample magnetometer (VSM), UV-vis., and fluorescence spectroscopy. The ternary phase behavior result shows varieties of aggregate structures achieved by changing the mixing ratios of the system components. The macroscopic visual appearance of the one phase aggregate changed from clear to bluish followed by more turbid, and show birefringent lamellar textures under cross polarizer's with increasing cholesterol mixing ratio with structural stability for more than one year. The vast majority of the results reveal assembly of vesicles displaying spherical morphology with diameters varying around 100 nm. Furthermore, the fluorescence anisotropy measurements show the transition of zwitterionic betaine micelles to vesicles structures promoted by cholesterol content; they have a significant effect on the rigidity and the size distribution of the vesicular dispersion. Additionally, the results of the influence of ion salts on vesicle dispersions show the ability of betaine vesicles to bind both cations and anions in order following the Hofmeister series, and exhibit interesting salt dependent properties with more preference to monovalent cations. Moreover, the results of hybrid assemble show that vesicles have been successful in embedding hydrophobic nanoparticles within their bilayer. The nanostructure of betaine vesicles act to concentrate small nanoparticles and provide a platform to shield and transport nanoparticles with appropriate stability to overcome of the shortcoming of individual nanoparticles delivery. Hybrid betaine nanovesicles can provide as model system into design of multifunctional nano-carriers for controlled and targeted drug delivery, with their potentials offering as diagnostic and therapeutic multimodality agents.,Certification of Master's/Doctoral Thesis" is not available |
| Pages: | 183 |
| Call Number: | QD431.E563 2017 tesis |
| Publisher: | UKM, Bangi |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_97907+SOURCE1+SOURCE1.0.PDF Restricted Access | 544.26 kB | Adobe PDF |  View/Open |
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