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https://ptsldigital.ukm.my/jspui/handle/123456789/499890| Title: | Composition studies of natural aroma chemicals from Malaysian Patchouli (Pogostemon cablin) and Agarwood (Aquilaria malaccensis) by detailed investigation of their raw materials, extracts and aroma fractions |
| Authors: | Norma Hussin (P53941) |
| Supervisor: | Mohd Ambar Yarmo, Prof. Dr. |
| Keywords: | Aroma chemicals Patchouli Agarwoods Fractionation Isolation Dissertations, Academic -- Malaysia |
| Issue Date: | 9-May-2016 |
| Description: | In this study, two woody crops namely Patchouli and Agarwoods have been focused for detailed investigations on the aspects of physical and chemical, aroma fractions, development of several new approaches of producing aroma materials from Patchouli and Agarwoods extracts. The justifications on these woody crops can be achieved based on its aroma characters due to the Patchouli crops being lacking in local aroma standards such as patchoulol for QC analysis. In the case of Agarwoods, in the present scenario we still have difficulties in interpreting its very compact sesquiterpenoid elutions when using a mono-dimensional gas chromatography (GCMS). Besides, there is still a lack of scientific evidence that needs to fully illustrate its aroma compound profiles. In Patchouli experiments, the aims of the study are to develop a simple technique in producing a premium grade patchouli alcohol ranging from 80-90% purity using simple fractionation and isolation techniques. This study shows that two patchoulol enantiomers can be well separated and identified as (-)-patchoulol (Rt 39.823 min) and (+)-patchoulol (Rt 40.94 min) by a GC-MS with a chiral column. Mixed enantiomers only eluted at Rt 40.00 min with a GC-MS non-polar column. The premium purity of patchoulol (compound I) was assessed to be 82% from P.cablin essential oil and can be refined up to 91% by a hand-made activated charcoal column treatment. The structure of the compound I was elucidated using GCMS, GC-FID and NMR to finally confirm it as (-)-patchoulol. On the other hand, detailed studies of Agarwoods crops presented impacts of technical physical characteristics of the raw materials and its parameters for value added essential oil products, evaluation using GCxGC-MS-FID to clearly verify Agarwoods compounds from the essential oil. Solid extraction (SE) method developed in this study revealed in the production of aromatic materials from Agarwood solid and liquid resins. Another aroma compound of interest, benzylacetone (4-phenyl-2-butanone, 4P2B) was successful intergrated by a GCxGC-MS-ToF profile together with other sesquiterpenes and fatty acids. A total of sixty constituents of Agarwoods aroma from the essential oils eluted in detail and correct RI with high compound similarity (80%) were obtained from GCxGC qMS analysis. In addition, two major esters were succesfully fractionated from the healthy wood extracts i.e. methyl hexadecanoate (25.8 %) and methyl 9(E)-octadecenoate (30.3%). A fraction of resinoid wood extract also gave 26.6% limonene. The fractions of natural aroma obtained from these studies have high potential to be commercialized and patented for Malaysia Agarwoods and Patchouli QC standards.,Certification of Master's/Doctoral Thesis" is not available |
| Pages: | 165 |
| Publisher: | UKM, Bangi |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_85409+SOURCE1+SOURCE1.0.PDF Restricted Access | 558.37 kB | Adobe PDF |  View/Open |
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