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https://ptsldigital.ukm.my/jspui/handle/123456789/499740Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Asmat Ahmad, Prof. Madya Dr. | - |
| dc.contributor.author | Zidan Abduldiem Bashir Abduldiem (P49250) | - |
| dc.date.accessioned | 2023-10-13T09:34:14Z | - |
| dc.date.available | 2023-10-13T09:34:14Z | - |
| dc.date.issued | 2016-04-15 | - |
| dc.identifier.other | ukmvital:82178 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/499740 | - |
| dc.description | Actinomycetes from marine environments may present rich sources of new bioactive compounds to combat clinically important pathogens. In the first part of this study, a total of 42 actinomycetes isolated from different marine sources in Malaysia were preliminarily screened for production of secondary metabolites was tested against several bacterial and fungal pathogens. The second part of this study aimed at evaluating the effects of seven nutritional and four environmental factors on the production of bioactive metabolites of a selected Streptomyces isolate in submerged culture using the Plackett-Burman design. Factors which significantly affected the activity of the metabolites produced were identified and statistically optimised by Response Surface Methodology (RSM) using a five-level, 30-run Box-Behnken design (BBD). Antimicrobial assays using two test organisms (Bacillus subtilis and Vibrio parahaemolyticus) and three anti-oxidant assays (CUPRAC, FRAP, DPPH) were incorporated to test for the activity of the produced metabolites. Out of all tested isolates, Streptomyces sp. PT1 isolate produced metabolites that demonstrated antimibrobial properties against all the tested microbes and was thus selected for further analysis. Glucose and yeast extract were found to be the most suitable carbon and nitrogen sources respectively, for the optimum production of bioactive metabolite by the isolate. One-way analysis of variance (ANOVA) of the responses showed that yeast extract, FeSO4, MnCl2 and NaCl were significant factors (p < 0.05) affecting the antimicrobial activity of secondary metabolite against B. subtilis and V. parahaemolyticus. For the metabolite produced by Streptomyces sp. PT1 in submerged culture, a combination of Plackett-Burman design and Box-Behnken Response Surface Methodology was found effective to optimize medium components of 66.05%, 43.46%, 13.96%, 26.80%, and 27.43% increase in response as compared to un-optimized medium using B. subtilis and V. parahaemolyticus as antimicrobial assays and antioxidant assays and FRAP, CUPRAC and DPPH as antioxidant assays, respectively. The study thus presents empirical proof of the biological activities of metabolites produced by Streptomyces sp. PT1 and that fermentation media could be optimised.,Certification of Master's/Doctoral Thesis" is not available | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Bangi | - |
| dc.relation | Faculty of Science and Technology / Fakulti Sains dan Teknologi | - |
| dc.rights | UKM | - |
| dc.subject | Streptomyces sp | - |
| dc.subject | Metabolites | - |
| dc.subject | Fermentation media | - |
| dc.subject | Marine sources | - |
| dc.subject | Dissertations, Academic -- Malaysia | - |
| dc.title | Marine streptomyces sp. Pt1 isolate as a potential source of bioactive secondary metabolites | - |
| dc.type | Theses | - |
| dc.format.pages | 181 | - |
| dc.identifier.barcode | 002293(2016) | - |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_82178+SOURCE1+SOURCE1.0.PDF Restricted Access | 304.53 kB | Adobe PDF |  View/Open |
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