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https://ptsldigital.ukm.my/jspui/handle/123456789/499486Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Salmijah Surif, Prof. Dr. | - |
| dc.contributor.author | Wong Kok Kee (P41727) | - |
| dc.date.accessioned | 2023-10-13T09:32:17Z | - |
| dc.date.available | 2023-10-13T09:32:17Z | - |
| dc.date.issued | 2012-01-03 | - |
| dc.identifier.other | ukmvital:74945 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/499486 | - |
| dc.description | A method to treat crude oil and heavy metals pollutants in the environment using a mixture of bacterial consortium acclimatised to heavy metals, and cyanobacteria was successfully developed. These heavy metal resistant bacterial consortium culture (CC) was able to utilise crude oil as sole carbon source for growth. The CC at 5% (v/v) inoculum size was found to be most successful in degrading crude oil compared to 1% (v/v) and 10% (v/v) inoculum sizes. The CC was found to degrade crude oil under the following conditions; pH 6.5 to 8.5 (optimum pH at 7.0) and temperature from 25°C to 55°C (optimum temperature at 30°C). Surface tension test proved the secretion of biosurfactant by the CC, used in the formation of micelle causing reduction in the culture media surface tension for up to 26.6%. Biochemical assays showed that the biosurfactant was from the glycolipid group. Preliminary study on the formation of microbial mat (consisting of CC and cyanobacteria) using two types of cyanobacteria, Selenastrum capricornutum and Anabaena tolurosa found that CC and S. capricornutum together were most successful in degrading crude oil. CC to S. capricornutum ratio of 5:1 gave the highest degradation of 1% (v/v) crude oil at 81.2% (p<0.05) within four weeks. The microbial mat was also found to be able to completely degrade a more polar hydrocarbon, i.e. phenol up to a maximum concentration of 1200 mg/L within 20 days. Degradation of phenol was catalysed by catechol-1,2-dioxygenase at 0.083 μmole-1mg-1protein, suggesting that other crude oil components with recalcitrant aromatic ring structure can be cleaved via similarly ortho-pathway to achieve complete degradation. Further, the ability of the microbial mat to degrade crude oil in the presence of lead (Pb) and cadmium (Cd) was studied. The microbial mat was able to degrade (0.4% v/v) crude oil up to 96% within eight days in the presence of relatively high concentrations of Pb (1.0 mg/L) and Cd (0.1 mg/L). The microbial mat was also shown able to simultaneously remove 82% - 98% of the metals from the culture media, possibly through bioaccumulation and bioadsorption. When the microbial mat was tested on the environmental effluent, it was shown to degrade 98.2% crude oil in 8 days and the growth and crude oil degradation ability of the microbial mat were not affected by the presence of the indigenous microbial population. The microbial mat was also shown able to remove metals 57.8% Cr, 72.4% Ni, 58.9% Cu, 67.9% Zn, 86.1% Cd and 89.2% Pb. The results from the study showed the developed microbial mat comprising of CC and S. capricornutum was able to degrade 96% of crude oil in the presence of Pb (1.0 mg/L) and Cd (0.1 mg/L), which are ten times higher than the permissible limit as stipulated by Environmental Quality Act 1974, Malaysia.,PhD | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Bangi | - |
| dc.relation | Faculty of Science and Technology / Fakulti Sains dan Teknologi | - |
| dc.rights | UKM | - |
| dc.subject | Microbial mat | - |
| dc.subject | Biological treatment | - |
| dc.subject | Crude oil contamination | - |
| dc.subject | Petroleum--Biodegradation | - |
| dc.title | Development of a microbial mat for the biological treatment of crude oil contamination | - |
| dc.type | Theses | - |
| dc.format.pages | 166 | - |
| dc.identifier.callno | TD427.P4 .W645 2012 | - |
| dc.identifier.barcode | 000183 | - |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_74945+Source01+Source010.PDF Restricted Access | 2.04 MB | Adobe PDF |  View/Open |
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